血管生成素（Ang）在特定TRNA的反逆转带内或附近切割，包括但不限于：TRNA ARG ACG（FU等人2009），TRNA ARG CCG（FU等人，2009），TRNA GLU CTC（FU等人。2009）Trna gly ccc（fu等人2009），trna gly gcc（fu等人2009），trna met cat（fu等人2009，su等，2019），trna pro agg（yamasaki等，2009），trna pro tgg（yamasaki等，2009），trna val aac（fu等人2009），trna Ala AGC（Su等人2019），Trna Ala CGC（Su等，2019），Trna Ala TGC（Su等人。2019年），TRNA ASP GTC（SU等人2019），TRNA GLU TTC（SU等人2019），TRNA他的GTG（SU等，2019），TRNA LEU CAG（SU等人2019），tRNA Leu TAG (Su et al. 2019), tRNA Lys TTT (Su et al. 2019), tRNA Ser GCT (Su et al. 2019), tRNA Ser CGA (Su et al. 2019), tRNA Val CAC (Su et al. 2019), tRNA Val TAC (Su et al. 2019) (also Lee and Vallee 1989, Saxena et al. 1992, Emara et al. 2010, Ivanov et al. 2011). The products are a 5' fragment of about 30-35 nt and a 3' fragment of about 40 nt known as tRNA halves or stress-induced tRNA fragments (tiRNAs) (Emara et al. 2010). As a result of ANG cleavage, the 5’ tRNA halves contain 5' monophosphates (Emara et al. 2010) and 3' cyclic monophosphates (Shigematsu et al. 2018), while the 3’ tRNA halves contain 5' hydroxyl groups (Shigematsu et al., 2018). ANG cleaves tRNA in response to biological conditions such as exposure to sex hormones and stresses such as starvation, oxidative stress, and virus infection (Fu et al. 2009, Emara et al. 2010, Ivanov et al. 2011, Wang et al. 2013, Honda et al. 2015, Selitsky et al. 2015), but several tRNA halves are still produced after stress in ANG knockout cells (Su et al. 2020). The 5' tiRNAs inhibit translation by displacing eIF4F from the m(7)G caps of mRNAs (Emara et al. 2010, Ivanov et al. 2011). The 3’ tiRNAs protect cells against stress-induced apoptosis by interacting with cytochrome C (inferred from mouse homologs in Saikia et al, 2014). The products of ANG have modifications present on mature tRNAs (Drino et al. 2020); therefore, the cleavage is believed to occur in the cytosol (Yamasaki et al. 2009, reviewed in Lyons et al. 2018) perhaps as ANG is translocated from receptors on the plasma membrane through the cytosol to the nucleus.