反式高尔基网络是来自内溶酶体系统和质膜的逆行货物的对接点。典型的货物包括回收居民TGN蛋白质如TGOLN2(也称为TGN46),如mannose-6-phosphate受体和受体像志贺毒素,霍乱和蓖麻毒素逆行贩卖机械的使用“搭便车”通过释放到细胞质分泌系统(审查在约翰和Popoff,2008;菲,2011;Sandvig等人,2013)。这些货物以网格蛋白和ap1依赖的方式从内吞系统运输,这在“反式高尔基网络出芽途径”中有更详细的描述(只是还没有)。一般来说,囊泡在其栓系和融合在TGN前是未被包裹的。在TGN中,至少存在2个不同的栓系通路。rab6依赖途径有助于早期内吞途径的囊泡融合和对接。这些囊泡,携带货物如TGOLN2和毒素,通过与TGN定位的Golgin系链以及与多亚基系链复合物COG和GARP的相互作用在TGN上停靠(Bonafacino和Rojas, 2006; Bonafacino and Hierro, 2011; Pfeffer, 2011). In contrast, mannose-6-phosphate receptors appear to traffic from late endosomes to the TGN through a RAB9- and PLIN3-dependent pathway. Vesicles are recruited to the TGN through interaction of RAB9 with the atypical RHO GTPase RHOBTB3, and tethered by virtue of interaction with TGN-localized Golgins and the GARP complex (Perez-Victoria et al, 2008; Perez-Victoria et al, 2009; Diaz et al, 1999; reviewed in Pfeffer, 2011; Chia and Gleeson, 2014)