BioPAX pathway converted from "GOT2 dimer transfers amino group from L-Cys to 2OG to form 3MPYR and Glu" in the Reactome database.
LEFT-TO-RIGHT
2.6.1.3
GOT2 dimer transfers amino group from L-Cys to 2OG to form 3MPYR and Glu
Hydrogen sulfide (H2S) produced endogenously has been established as the third gaseous signaling molecule, a smooth muscle relaxant and a neuroprotectant (Kimura 2011a, 2011b). Three human enzyme systems produce H2S in the brain, retina and vascular endothelial cells. 3-mercaptopyruvate sulphurtransferase (MPST, aka 3MST) in conjunction with cysteine (aspartate) aminotransferase (CAT, aka GOT2) is decribed here. The first step is the reversible transamination between L-cysteine (L-Cys) and 2-oxoglutarate (2OG, aka alpha-ketoglutarate) to form 3-methylpyruvate (3MPYR) and glutamate (Glu) catalysed by GOT2. Two forms of human aspartate aminotransferase (GOT) enzymes exist; cytosolic (GOT1) and mitochondrial (GOT2). Both are dimeric proteins requiring pyridoxal phosphate for activity. Human GOT2 (Zhou et al. 1998) possesses the same catalytic activity as its rat counterpart (Ubuka et al. 1978).
Authored: Jassal, Bijay, 2017-07-17
Reviewed: Kimura, Hideo, 2018-01-11
编辑:Jassal Bijay, 2017-07-17
2OG
2-Oxoglutarate
2-oxoglutaric acid
Oxoglutaric acid
2-Ketoglutaric acid
alpha-Ketoglutaric acid
2-oxoglutarate(2-)
Reactome DB_ID: 113594
mitochondrial matrix
GENE ONTOLOGY
GO:0005759
2-oxoglutarate(2-) [ChEBI:16810]
2-oxoglutarate(2-)
2-ketoglutarate
alpha-ketoglutarate
2-oxopentanedioic acid, ion(2-)
2-oxoglutarate
ChEBI
CHEBI:16810
Reactome Database ID Release 77
113594
Database identifier. Use this URL to connect to the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=113594
Reactome
R-ALL-113594
4
Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser_st_id?ST_ID=R-ALL-113594.4
Reactome
//www.joaskin.com
COMPOUND
C00026
additional information
MI
小姐:0361
L-Cys
L-2-amino-3-mercaptopropionic acid
L-cysteine zwitterion
L-cysteine
Reactome DB_ID: 379721
L-cysteine zwitterion [ChEBI:35235]
L-cysteine zwitterion
C3H7NO2S
(2R)-2-ammonio-3-sulfanylpropanoate
121.15922
[NH3+][C@@H](CS)C([O-])=O
L-cysteine
InChI=1S/C3H7NO2S/c4-2(1-7)3(5)6/h2,7H,1,4H2,(H,5,6)/t2-/m0/s1
(2R)-2-ammonio-3-mercaptopropanoate
XUJNEKJLAYXESH-REOHCLBHSA-N
ChEBI
CHEBI:35235
Reactome Database ID Release 77
379721
Database identifier. Use this URL to connect to the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=379721
Reactome
R-ALL-379721
4
Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser_st_id?ST_ID=R-ALL-379721.4
COMPOUND
C00097
3MPYR
3-mercaptopyruvate
3-mercaptopyruvic acid
Reactome DB_ID: 9012592
3-mercaptopyruvic acid [ChEBI:16208]
3-mercaptopyruvic acid
ChEBI
CHEBI:16208
Reactome Database ID Release 77
9012592
Database identifier. Use this URL to connect to the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=9012592
Reactome
R-ALL-9012592
3
Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser_st_id?ST_ID=R-ALL-9012592.3
Glu
L-Glu
L-glutamate(1-)
L-glutamic acid
L-Glutamate
L-Glutaminic acid
Reactome DB_ID: 113552
L-glutamate(1-) [ChEBI:29985]
L-glutamate(1-)
L-glutamic acid, ion(1-)
[NH3+][C@@H](CCC([O-])=O)C([O-])=O
WHUUTDBJXJRKMK-VKHMYHEASA-M
C5H8NO4
146.12136
(2S)-2-ammoniopentanedioate
hydrogen L-glutamate
L-glutamate
L-glutamic acid monoanion
InChI=1S/C5H9NO4/c6-3(5(9)10)1-2-4(7)8/h3H,1-2,6H2,(H,7,8)(H,9,10)/p-1/t3-/m0/s1
ChEBI
CHEBI:29985
Reactome Database ID Release 77
113552
Database identifier. Use this URL to connect to the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=113552
Reactome
R-ALL-113552
5
Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser_st_id?ST_ID=R-ALL-113552.5
COMPOUND
C00025
ACTIVATION
GOT2 dimer
aspartate aminotransferase, mitochondrial, holoenzyme
Reactome DB_ID: 70594
GOT2
PXLP-K279-GOT2
aspartate aminotransferase, mitochondrial, apoenzyme monomer
glutamate oxaloacetate transaminase-2
Aspartate aminotransferase, mitochondrial precursor
Transaminase A
Reactome DB_ID: 9608413
UniProt:P00505 GOT2
GOT2
KYAT4
催化功能不可逆的转氨作用of the L-tryptophan metabolite L-kynurenine to form kynurenic acid (KA). As a member of the malate-aspartate shuttle, it has a key role in the intracellular NAD(H) redox balance. Is important for metabolite exchange between mitochondria and cytosol, and for amino acid metabolism. Facilitates cellular uptake of long-chain free fatty acids.SUBUNIT Homodimer.INDUCTION Up-regulated by long-time exposure to alcohol.MISCELLANEOUS In eukaryotes there are cytoplasmic, mitochondrial and chloroplastic isozymes.SIMILARITY Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.
Homo sapiens
NCBI Taxonomy
9606
UniProt
P00505
279
EQUAL
N6-pyridoxal phosphate-L-lysine
MOD
MOD:00128
30
EQUAL
430
EQUAL
Reactome Database ID Release 77
9608413
Database identifier. Use this URL to connect to the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=9608413
Reactome
R-HSA-9608413
2
Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser_st_id?ST_ID=R-HSA-9608413.2
2
Reactome Database ID Release 77
70594
Database identifier. Use this URL to connect to the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=70594
Reactome
R-HSA-70594
2
Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser_st_id?ST_ID=R-HSA-70594.2
GENE ONTOLOGY
GO:0047801
gene ontology term for cellular function
MI
MI:0355
Same Catalyst Activity
Reactome Database ID Release 77
9012605
Database identifier. Use this URL to connect to the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=9012605
Reactome Database ID Release 77
9012597
Database identifier. Use this URL to connect to the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=9012597
Reactome
R-HSA-9012597
1
Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser_st_id?ST_ID=R-HSA-9012597.1
9537447
Pubmed
1998
Ethanol up-regulates fatty acid uptake and plasma membrane expression and export of mitochondrial aspartate aminotransferase in HepG2 cells
Zhou, S L
Gordon, R E
Bradbury, M
Stump, D
Kiang, C L
Berk, P D
Hepatology 27:1064-74
20191298
Pubmed
2011
Hydrogen sulfide: its production, release and functions
Kimura, Hideo
Amino Acids 41:113-21
754189
Pubmed
1978
Purification and characterization of mitochondrial cysteine aminotransferase from rat liver
Ubuka, T
Umemura, S
Yuasa, S
Kinuta, M
Watanabe, K
Physiol. Chem. Phys. 10:483-500
21527544
Pubmed
2011
Hydrogen sulfide: its production and functions
Kimura, Hideo
Exp. Physiol. 96:833-5