BioPAX pathway converted from "rRNA modification in the mitochondrion" in the Reactome database.
rRNA modification in the mitochondrion
Five modified nucleotides have been detected in the 12S rRNA: 5-methylcytidine-841 catalyzed by NSUN4, 6-dimethyladenosine-936 catalyzed by TFB1M, 6-dimethyladenosine-937 catalyzed by TFB1M, 5-methyluridine-429, and 4-methylcytidine-839 (reviewed in Van Haute et al. 2015). Four modified nucleotides have been detected in 16S rRNA: 2'-O-methylguanosine-1145 catalyzed by MRM1, 2'-O-methylguanosine-1370 catalyzed by RNMTL1 (MRM3), 2'-O-methyluridine-1369 catalyzed by FTSJ2 (MRM2), and pseudouridine-1397. 2'-O-methyluridine-1369 and 2'-O-methylguanosone-1370 occur in the A-loop of rRNA which is located at the peptidyl transferase center of the large subunit. Here the modified residues play a role in interaction with the aminoacyl site of tRNA. Knockouts of TFB1M and NSUN4 are lethal in mice and mutations in TFB1M may be related to aminoglycoside-induced deafness (reviewed in Van Haute et al. 2015).
Authored: May, Bruce, 2015-08-28
Reviewed: Bogenhagen, Daniel F, 2016-04-25
Edited: May, Bruce, 2015-08-28
LEFT-TO-RIGHT
2.1.1
NSUN4 methylates cytidine-841 of 12S rRNA yielding 5-methylcytidine-841
MTERF4 forms a complex with NSUN4 (Camara et al. 2011, Spahr et al. 2012, Yakubovskaya et al. 2012) and recruits NSUN4 to the ribosome where NSUN4 transfers a methyl group from S-adenosylmethionine to the 5 position of cytidine-841 in 12S rRNA (Camara et al. 2011). Knockout of MTERF4 in mice is lethal and cardiomyocyte-specific knockout is accompanied by mitochondrial cardiomyopathy (Camara et al. 2011).
Authored: May, Bruce, 2015-08-28
Reviewed: Bogenhagen, Daniel F, 2016-04-25
Edited: May, Bruce, 2015-08-28
SAM
AdoMet
S-adenosyl-L-methionine
S-adenosylmethionine
Reactome DB_ID: 2162265
mitochondrial matrix
GENE ONTOLOGY
GO:0005759
S-adenosyl-L-methionine [ChEBI:15414]
S-adenosyl-L-methionine
ChEBI
CHEBI:15414
Reactome Database ID Release 77
2162265
Database identifier. Use this URL to connect to the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=2162265
Reactome
R-ALL-2162265
5
Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser_st_id?ST_ID=R-ALL-2162265.5
Reactome
//www.joaskin.com
MT-RNR1
12S rRNA
Reactome DB_ID: 6792974
ENSEMBL:ENST00000389680 MT-RNR1
Mitochondrial 12S rRNA
MT-RNR1
Homo sapiens
NCBI Taxonomy
9606
ENSEMBL
ENST00000389680
Reactome Database ID Release 77
6792974
Database identifier. Use this URL to connect to the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=6792974
Reactome
R-HSA-6792974
1
Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser_st_id?ST_ID=R-HSA-6792974.1
SAH
AdoHcy
S-adenosyl-L-homocysteine
S-adenosylhomocysteine
Reactome DB_ID: 2162252
S-adenosyl-L-homocysteine [ChEBI:16680]
S-adenosyl-L-homocysteine
ChEBI
CHEBI:16680
Reactome Database ID Release 77
2162252
Database identifier. Use this URL to connect to the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=2162252
Reactome
R-ALL-2162252
4
Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser_st_id?ST_ID=R-ALL-2162252.4
MT-RNR1
12S rRNA containing 5-methylC-841
12S rRNA containing 5-methylcytosine-841
Reactome DB_ID: 6793078
Reactome Database ID Release 77
6793078
Database identifier. Use this URL to connect to the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=6793078
Reactome
R-HSA-6793078
2
Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser_st_id?ST_ID=R-HSA-6793078.2
ChEBI
74483
additional information
MI
MI:0361
ACTIVATION
activeUnit: #实验研究
MTERF4:NSUN4
Reactome DB_ID: 6793090
MTERF4
Transcription termination factor 4, mitochondrial
MTEF4_HUMAN
Reactome DB_ID: 6793089
UniProt:Q7Z6M4 MTERF4
MTERF4
MTERFD2
HSPC096
FUNCTION Regulator of mitochondrial ribosome biogenesis and translation. Binds to mitochondrial ribosomal RNAs 16S, 12S and 7S and targets NSUN4 RNA methyltransferase to the mitochondrial large ribosomal subunit (39S).SUBUNIT Heterodimer with NSUN4; this interaction may be required for NSUN4 recruitment to the mitochondrial large ribosomal subunit.DOMAIN The MTERF repeats form a half-donut shaped, right-handed superhelix, where the concave side displays a positively charged path for nucleic acid interaction.PTM The mature mitochondrial protein exists in 2 forms differing at the level of their N-terminus, one is starting at residue 43 and the other at residue 48.SIMILARITY Belongs to the mTERF family.
UniProt
Q7Z6M4
43
EQUAL
381
EQUAL
Reactome Database ID Release 77
6793089
Database identifier. Use this URL to connect to the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=6793089
Reactome
R-HSA-6793089
2
Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser_st_id?ST_ID=R-HSA-6793089.2
1
NSUN4
5-methylcytosine rRNA methyltransferase NSUN4 ecNumber2.1.1.-/ecNumber
NSUN4_HUMAN
Reactome DB_ID: 6793068
UniProt:Q96CB9 NSUN4
NSUN4
FUNCTION Involved in mitochondrial ribosome assembly. 5-methylcytosine rRNA methyltransferase that probably is involved in mitochondrial ribosome small subunit (SSU) maturation by methylation of mitochondrial 12S rRNA; the function is independent of MTERFD2/MTERF4 and assembled mitochondrial ribosome large subunit (LSU). Targeted to LSU by MTERFD2/MTERF4 and probably is involved in a final step in ribosome biogenesis to ensure that SSU and LSU are assembled. In vitro can methylate 16S rRNA of the LSU; the methylation is enhanced by MTERFD/MTERF4.SIMILARITY Belongs to the class I-like SAM-binding methyltransferase superfamily. RsmB/NOP family.
UniProt
Q96CB9
26
EQUAL
384
EQUAL
Reactome Database ID Release 77
6793068
Database identifier. Use this URL to connect to the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=6793068
Reactome
R-HSA-6793068
2
Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser_st_id?ST_ID=R-HSA-6793068.2
1
Reactome Database ID Release 77
6793090
Database identifier. Use this URL to connect to the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=6793090
Reactome
R-HSA-6793090
2
Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser_st_id?ST_ID=R-HSA-6793090.2
GENE ONTOLOGY
GO:0009383
gene ontology term for cellular function
MI
MI:0355
Same Catalyst Activity
Reactome Database ID Release 77
6793074
Database identifier. Use this URL to connect to the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=6793074
Reactome Database ID Release 77
6793057
Database identifier. Use this URL to connect to the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=6793057
Reactome
R-HSA-6793057
3
Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser_st_id?ST_ID=R-HSA-6793057.3
23022348
Pubmed
2012
Structure of the essential MTERF4:NSUN4 protein complex reveals how an MTERF protein collaborates to facilitate rRNA modification
Yakubovskaya, Elena
Guja, Kip E
Mejia, Edison
Castano, Steven
Hambardjieva, Elena
Choi, Woo Suk
Garcia-Diaz, Miguel
Structure 20:1940-7
21531335
Pubmed
2011
MTERF4 regulates translation by targeting the methyltransferase NSUN4 to the mammalian mitochondrial ribosome
Cámara, Yolanda
Asin-Cayuela, Jorge
Park, Chan Bae
Metodiev Metodi D
Shi, Yonghong
Ruzzenente, Benedetta
Kukat, Christian
Habermann, Bianca
Wibom, Rolf
Hultenby, Kjell
Franz, Thomas
Erdjument-Bromage, H
Tempst, P
Hallberg, B Martin
Gustafsson, CM
Larsson, Nils-Göran
Cell Metab. 13:527-39
22949673
Pubmed
2012
Structure of the human MTERF4-NSUN4 protein complex that regulates mitochondrial ribosome biogenesis
Spåhr, Henrik
Habermann, Bianca
Gustafsson, CM
Larsson, Nils-Göran
Hallberg, B Martin
Proc. Natl. Acad. Sci. U.S.A. 109:15253-8
GENE ONTOLOGY
GO:0031167
gene ontology term for cellular process
MI
MI:0359
LEFT-TO-RIGHT
2.1.1
TFB1M dimethylates adenosine-936 and adenosine-937 of 12S rRNA yielding 6-dimethyladenosine-936 and 6-dimethyladenosine-937
TFB1M transfers methyl groups from S-adenosylmethionine to the 6 position of adenosine residues at nucleotides 936 and 937 in 12S mitochondrial rRNA (McCulloch et al. 2002, Seidel-Rogol et al. 2003, Cotney et al. 2009, Guja et al. 2013). The mitochondrial RNA polymerase POLRMT associates directly with TFB1M and increases its methylase activity (Surovtseva and Shadel 2013). Alleles of TFB1M modify the severity of hearing loss associated with the A1555G mutation in 12S rRNA (Bykhovskaya et al. 2004). The A1555G mutation may cause hypermethylation of 12S rRNA which activates AMP kinase, E2F1, and apoptosis (Cotney et al. 2009, Raimundo et al. 2012), however the reported hypermethylation and deafness phenotype have been questioned (Lee et al. 2015). Disruption of Tfb1m in mice is lethal and causes loss of adenine methylation of the 12S RNA (Metodiev et al. 2009).
Authored: May, Bruce, 2015-08-28
Reviewed: Bogenhagen, Daniel F, 2016-04-25
Edited: May, Bruce, 2015-08-28
4
4
MT-RNR1
12S rRNA containing 6-dimethylA-936,937
12S rRNA containing 6-dimethyladenosine-936 and 6-dimethyladenosine-937
Reactome DB_ID: 6793060
Reactome Database ID Release 77
6793060
Database identifier. Use this URL to connect to the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=6793060
Reactome
R-HSA-6793060
2
Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser_st_id?ST_ID=R-HSA-6793060.2
ACTIVATION
TFB1M
Dimethyladenosine transferase 1, mitochondrial
TFB1M_HUMAN
Reactome DB_ID: 1592207
UniProt:Q8WVM0 TFB1M
TFB1M
CGI-75
FUNCTION S-adenosyl-L-methionine-dependent methyltransferase which specifically dimethylates mitochondrial 12S rRNA at the conserved stem loop. Also required for basal transcription of mitochondrial DNA, probably via its interaction with POLRMT and TFAM. Stimulates transcription independently of the methyltransferase activity.SUBUNIT Interacts with mitochondrial RNA polymerase POLRMT. Interacts with TFAM.TISSUE SPECIFICITY Ubiquitously expressed.INDUCTION By the nuclear respiratory factors NRF1 and NRF2/GABPB2 and PGC-1 coactivators.DISEASE Variations in TFB1M may influence the clinical expression of aminoglycoside-induced deafness caused by the A1555G mutation in the mitochondrial 12S rRNA.SIMILARITY Belongs to the class I-like SAM-binding methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. KsgA subfamily.
UniProt
Q8WVM0
28
EQUAL
346
EQUAL
Reactome Database ID Release 77
1592207
Database identifier. Use this URL to connect to the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=1592207
Reactome
R-HSA-1592207
1
Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser_st_id?ST_ID=R-HSA-1592207.1
GENE ONTOLOGY
GO:0000179
Reactome Database ID Release 77
6793073
Database identifier. Use this URL to connect to the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=6793073
Reactome Database ID Release 77
6793066
Database identifier. Use this URL to connect to the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=6793066
Reactome
R-HSA-6793066
3
Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser_st_id?ST_ID=R-HSA-6793066.3
23804760
Pubmed
2013
Structural basis for S-adenosylmethionine binding and methyltransferase activity by mitochondrial transcription factor B1
Guja, Kip E
Venkataraman, Krithika
Yakubovskaya, Elena
Shi, Hui
Mejia, Edison
Hambardjieva, Elena
Karzai, A Wali
Garcia-Diaz, Miguel
Nucleic Acids Res. 41:7947-59
11809803
Pubmed
2002
A human mitochondrial transcription factor is related to RNA adenine methyltransferases and binds S-adenosylmethionine
McCulloch, V
Seidel-Rogol, BL
Shadel, GS
Mol Cell Biol 22:1116-25
22341444
Pubmed
2012
Mitochondrial stress engages E2F1 apoptotic signaling to cause deafness
Raimundo, Nuno
Song, Lei
Shutt, Timothy E
McKay, Sharen E
Cotney, Justin
Guan, Min-Xin
Gilliland, Thomas C
Hohuan, David
Santos-Sacchi, Joseph
Shadel, Gerald S
Cell 148:716-26
19356719
Pubmed
2009
12 s rRNA的甲基化是体内所必需的tability of the small subunit of the mammalian mitochondrial ribosome
Metodiev Metodi D
Lesko, Nicole
Park, Chan Bae
Cámara, Yolanda
Shi, Yonghong
Wibom, Rolf
Hultenby, Kjell
Gustafsson, CM
Larsson, Nils-Göran
Cell Metab. 9:386-97
26464487
Pubmed
2015
Overexpression of the mitochondrial methyltransferase TFB1M in the mouse does not impact mitoribosomal methylation status or hearing
Lee, Seungmin
Rose, Simon
Metodiev Metodi D
Becker, Lore
Vernaleken, Alexandra
Klopstock, Thomas
Gailus-Durner, Valerie
Fuchs, Helmut
Hrabě De Angelis, Martin
Douthwaite, Stephen
Larsson, Nils-Göran
Hum. Mol. Genet. 24:7286-94
23303773
Pubmed
2013
Transcription-independent role for human mitochondrial RNA polymerase in mitochondrial ribosome biogenesis
Surovtseva, Yulia V
Shadel, Gerald S
Nucleic Acids Res. 41:2479-88
19417006
Pubmed
2009
Elucidation of separate, but collaborative functions of the rRNA methyltransferase-related human mitochondrial transcription factors B1 and B2 in mitochondrial biogenesis reveals new insight into maternally inherited deafness
Cotney, Justin
McKay, Sharen E
Shadel, Gerald S
Hum. Mol. Genet. 18:2670-82
12496758
Pubmed
2003
Human mitochondrial transcription factor B1 methylates ribosomal RNA at a conserved stem-loop
Seidel-Rogol, BL
McCulloch, V
Shadel, GS
Nat Genet 33:23-4
15110318
Pubmed
2004
Human mitochondrial transcription factor B1 as a modifier gene for hearing loss associated with the mitochondrial A1555G mutation
Bykhovskaya, Yelena
Mengesha, Emebet
Wang, Dai
Yang, Huiying
Estivill, X
Shohat, Mordechai
Fischel-Ghodsian, Nathan
Mol. Genet. Metab. 82:27-32
LEFT-TO-RIGHT
MRM3 (RNMTL1) methylates guanosine-1370 of 16S rRNA yielding 2'-O-methylguanosine-1370
MRM3 (RNMTL1) associates with the large mitochondrial ribosomal subunit and transfers a methyl group from S-adenosylmethionine to the 2' hydroxyl group of guanosine-1370 of 16S rRNA (Lee et al. 2013, Lee and Bogenhagen 2014). Inactivation of RNMTL1 causes aberrant assembly of large ribosomal subunits, decreased mitochondrial translation, and respiratory incompetence (Rorbach et al. 2014).
Authored: May, Bruce, 2015-08-29
Reviewed: Bogenhagen, Daniel F, 2016-04-25
Edited: May, Bruce, 2015-08-29
MT-RNR2
16S rRNA
Reactome DB_ID: 6792998
ENSEMBL:ENST00000387347 MT-RNR2
Mitochondrial 16S rRNA
MT-RNR2
ENSEMBL
ENST00000387347
Reactome Database ID Release 77
6792998
Database identifier. Use this URL to connect to the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=6792998
Reactome
R-HSA-6792998
1
Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser_st_id?ST_ID=R-HSA-6792998.1
MT-RNR2
16S rRNA containing 2'-O-methylG-1370
16S rRNA containing 2'-O-methylguanosine-1370
Reactome DB_ID: 6793086
Reactome Database ID Release 77
6793086
Database identifier. Use this URL to connect to the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=6793086
Reactome
R-HSA-6793086
2
Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser_st_id?ST_ID=R-HSA-6793086.2
ChEBI
74445
ACTIVATION
RNMTL1
fullName evidence="9"rRNA methyltransferase 3, mitochondrial ecNumber evidence="12 13"2.1.1.-/ecNumber
MRM3_HUMAN
Reactome DB_ID: 6793067
UniProt:Q9HC36 MRM3
MRM3
RNMTL1
HC90
-O-ri函数S-adenosyl-L-methionine-dependent 2”bose methyltransferase that catalyzes the formation of 2'-O-methylguanosine at position 1370 (Gm1370) in the 16S mitochondrial large subunit ribosomal RNA (mtLSU rRNA), a conserved modification in the peptidyl transferase domain of the mtLSU rRNA.TISSUE SPECIFICITY Expressed at same level in normal liver and hepatocarcinoma.SIMILARITY Belongs to the class IV-like SAM-binding methyltransferase superfamily. RNA methyltransferase TrmH family.
UniProt
Q9HC36
41
EQUAL
420
EQUAL
Reactome Database ID Release 77
6793067
Database identifier. Use this URL to connect to the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=6793067
Reactome
R-HSA-6793067
2
Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser_st_id?ST_ID=R-HSA-6793067.2
GENE ONTOLOGY
GO:0070039
Reactome Database ID Release 77
6793094
Database identifier. Use this URL to connect to the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=6793094
Reactome Database ID Release 77
6793096
Database identifier. Use this URL to connect to the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=6793096
Reactome
R-HSA-6793096
3
Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser_st_id?ST_ID=R-HSA-6793096.3
24036117
Pubmed
2013
Mitochondrial ribosomal RNA (rRNA) methyltransferase family members are positioned to modify nascent rRNA in foci near the mitochondrial DNA nucleoid
Lee, Ken-Wing
Okot-Kotber, Cynthia
LaComb, Joseph F
Bogenhagen, Daniel F
J. Biol. Chem. 288:31386-99
25009282
Pubmed
2014
MRM2 and MRM3 are involved in biogenesis of the large subunit of the mitochondrial ribosome
Rorbach, Joanna
Boesch, Pierre
Gammage, Payam A
Nicholls, Thomas J J
Pearce, Sarah F
Patel, Dipali
Hauser, Andreas
Perocchi, Fabiana
Minczuk, Michal
Mol. Biol. Cell 25:2542-55
25074936
Pubmed
2014
Assignment of 2'-O-methyltransferases to modification sites on the mammalian mitochondrial large subunit 16 S ribosomal RNA (rRNA)
Lee, Ken-Wing
Bogenhagen, Daniel F
J. Biol. Chem. 289:24936-42
GENE ONTOLOGY
GO:0000451
LEFT-TO-RIGHT
2.1.1
MRM2 (FTSJ2) methylates uridine-1369 of 16S rRNA yielding 2'-O-methyluridine
MRM2 (FTSJ2)位于线粒体ν附近的焦点cleoids (Lee et al. 2013) transfers a methyl group from S-adenosylmethionine to the 2' hydroxyl of uridine-1369 in 16S rRNA (Lee and Bogenhagen 2014, Rorbach et al. 2014). Inactivation of FTSJ2 causes aberrant assembly of large mitochondrial subunits, reduced mitochondrial translation, and respiratory incompetence (Rorbach et al. 2014).
Authored: May, Bruce, 2015-08-30
Reviewed: Bogenhagen, Daniel F, 2016-04-25
Edited: May, Bruce, 2015-08-30
MT-RNR2
16S rRNA containing 2'-O-methylU-1369
16S rRNAcontaining 2'-O-methyluridine-1369
Reactome DB_ID: 6793117
Reactome Database ID Release 77
6793117
Database identifier. Use this URL to connect to the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=6793117
Reactome
R-HSA-6793117
2
Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser_st_id?ST_ID=R-HSA-6793117.2
ChEBI
74478
ACTIVATION
FTSJ2
fullName evidence="9"rRNA methyltransferase 2, mitochondrial ecNumber evidence="13"2.1.1.-/ecNumber
MRM2_HUMAN
Reactome DB_ID: 6793061
UniProt:Q9UI43 MRM2
MRM2
FJH1
FTSJ2
-O-ri函数S-adenosyl-L-methionine-dependent 2”bose methyltransferase that catalyzes the formation of 2'-O-methyluridine at position 1369 (Um1369) in the 16S mitochondrial large subunit ribosomal RNA (mtLSU rRNA), a universally conserved modification in the peptidyl transferase domain of the mtLSU rRNA.TISSUE SPECIFICITY Widely expressed, with highest expression in muscle, placenta, and heart.SIMILARITY Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA methyltransferase RlmE family.
UniProt
Q9UI43
19
EQUAL
246
EQUAL
Reactome Database ID Release 77
6793061
Database identifier. Use this URL to connect to the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=6793061
Reactome
R-HSA-6793061
2
Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser_st_id?ST_ID=R-HSA-6793061.2
GENE ONTOLOGY
GO:0008650
Reactome Database ID Release 77
6793126
Database identifier. Use this URL to connect to the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=6793126
Reactome Database ID Release 77
6793127
Database identifier. Use this URL to connect to the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=6793127
Reactome
R-HSA-6793127
3
Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser_st_id?ST_ID=R-HSA-6793127.3
LEFT-TO-RIGHT
MRM1 methylates guanosine-1145 of 16S rRNA yielding 2'-O-methylguanosine-1145
MRM1位于线粒体类核附近的焦点(Lee et al. 2013) transfers a methyl group from S-adenosylmethionine to the 2' hydroxyl of guanosine-1145 in 16S rRNA (Lee and Bogenhagen 2014).
Authored: May, Bruce, 2015-08-30
Reviewed: Bogenhagen, Daniel F, 2016-04-25
Edited: May, Bruce, 2015-08-30
MT-RNR2
16S rRNA containing 2'-O-methylG-1145
16S rRNA containing 2'-O-methylguanosine-1145
Reactome DB_ID: 6793123
Reactome Database ID Release 77
6793123
Database identifier. Use this URL to connect to the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=6793123
Reactome
R-HSA-6793123
2
Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser_st_id?ST_ID=R-HSA-6793123.2
ChEBI
74445
ACTIVATION
MRM1
fullName evidence="7"rRNA methyltransferase 1, mitochondrial ecNumber evidence="11"2.1.1.-/ecNumber
MRM1_HUMAN
Reactome DB_ID: 6793088
UniProt:Q6IN84 MRM1
MRM1
-O-ri函数S-adenosyl-L-methionine-dependent 2”bose methyltransferase that catalyzes the formation of 2'-O-methylguanosine at position 1145 (Gm1145) in the 16S mitochondrial large subunit ribosomal RNA (mtLSU rRNA), a universally conserved modification in the peptidyl transferase domain of the mtLSU rRNA.SIMILARITY Belongs to the class IV-like SAM-binding methyltransferase superfamily. RNA methyltransferase TrmH family.
UniProt
Q6IN84
21
EQUAL
353
EQUAL
Reactome Database ID Release 77
6793088
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Reactome
R-HSA-6793088
2
Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser_st_id?ST_ID=R-HSA-6793088.2
Reactome Database ID Release 77
6793124
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Reactome Database ID Release 77
6793122
Database identifier. Use this URL to connect to the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=6793122
Reactome
R-HSA-6793122
3
Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser_st_id?ST_ID=R-HSA-6793122.3
Reactome Database ID Release 77
6793080
Database identifier. Use this URL to connect to the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=6793080
Reactome
R-HSA-6793080
3
Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser_st_id?ST_ID=R-HSA-6793080.3
26016801
Pubmed
2015
Mitochondrial transcript maturation and its disorders
Van Haute, Lindsey
Pearce, Sarah F
Powell, Christopher A
D'Souza, Aaron R
Nicholls, Thomas J
Minczuk, Michal
J. Inherit. Metab. Dis. 38:655-80
22642575
Pubmed
2012
The post-transcriptional life of mammalian mitochondrial RNA
Rorbach, Joanna
Minczuk, Michal
Biochem. J. 444:357-73
GENE ONTOLOGY
GO:0000154