BioPAX途径由Reactome数据库中的“carbovir二磷酸+ ATP => carbovir三磷酸+ ADP”转化而来。 从左到右 2.7.4.6 二磷酸卡韦的磷酸化 二磷酸卡韦+ ATP =>三磷酸卡韦+ ADP 胞质PCK1(磷酸烯醇丙酮酸羧激酶1)催化二磷酸卡韦与ATP反应生成卡韦三磷酸和ADP。值得注意的是，大鼠PCK1在体外催化该反应的速率大约是牛核苷二磷酸激酶的70倍(Miller等，1992年)。 作者:D’eustachio, P, 2012-03-14 评论:Jassal, B, 2012-03-16 编辑:D’eustachio, P, 2012-03-16 异型生物质 carbovir二磷酸 Reactome DB_ID: 2162075 胞质 基因本体论 去:0005829 (PubChem化合物:135564825) PubChem化合物 135564825 Reactome数据库ID Release 77 2162075 数据库标识符。使用此URL连接到Reactome中的此实例的网页://www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=2162075 Reactome r - - 2162075 3. Reactome稳定的标识符。使用此URL连接到Reactome中的此实例的网页://www.joaskin.com/cgi-bin/eventbrowser_st_id?ST_ID=R-ALL-2162075.3 Reactome //www.joaskin.com 三磷酸腺苷 腺苷5 '三磷酸 ATP (4) Reactome DB_ID: 113592 ATP (4 -) (ChEBI: 30616) ATP (4) 三磷酸腺苷 三磷酸腺苷 腺苷5 '三磷酸 ChEBI CHEBI: 30616 Reactome数据库ID Release 77 113592 数据库标识符。使用此URL连接到Reactome中的此实例的网页://www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=113592 Reactome r - - 113592 5 Reactome稳定的标识符。使用此URL连接到Reactome中的此实例的网页://www.joaskin.com/cgi-bin/eventbrowser_st_id?ST_ID=R-ALL-113592.5 复合 C00002 额外的信息 心肌梗死 小姐:0361 ADP 腺苷5 '磷酸氢盐 ADP (3 -) Reactome DB_ID: 29370 ADP (3 -) (ChEBI: 456216) ADP (3 -) ADP 事情就让它5,- o - [(phosphonatooxy) phosphinato]腺苷 ADP trianion ChEBI CHEBI: 456216 Reactome数据库ID Release 77 29370 数据库标识符。使用此URL连接到Reactome中的此实例的网页://www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=29370 Reactome r - - 29370 5 Reactome稳定的标识符。使用此URL连接到Reactome中的此实例的网页://www.joaskin.com/cgi-bin/eventbrowser_st_id?ST_ID=R-ALL-29370.5 复合 C00008 异型生物质 carbovir三磷酸 Reactome DB_ID: 2162091 (PubChem化合物:135499583) PubChem化合物 135499583 Reactome数据库ID Release 77 2162091 数据库标识符。使用此URL连接到Reactome中的此实例的网页://www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=2162091 Reactome r - - 2162091 3. Reactome稳定的标识符。使用此URL连接到Reactome中的此实例的网页://www.joaskin.com/cgi-bin/eventbrowser_st_id?ST_ID=R-ALL-2162091.3 激活 Pck1 胞质磷酸烯醇丙酮酸羧激酶 Reactome DB_ID: 2161508 UniProt: P07379 Pck1 Pck1 功能:Cytosolic phosphoenolpyruvate carboxykinase催化草酰乙酸(OAA)可逆脱羧和磷酸化，并作为糖异生的限速酶(PubMed:4186849, PubMed:30193097, PubMed:26322521, PubMed:26709450, PubMed:28345895, PubMed:31461616)。调节退化和异变，这一过程控制柠檬酸循环中代谢中间体的水平(PubMed:30193097)。在低葡萄糖水平下，它催化草酰乙酸分解转化为磷酸烯醇丙酮酸(PEP)，这是代谢途径中的限速步骤，从乳酸和来自柠檬酸循环的其他前体产生葡萄糖(PubMed:30193097)。在高葡萄糖水平下，它催化磷酸烯醇丙酮酸转化为草酰乙酸(PubMed:30193097)。作为记忆性CD8(+) t细胞的形成和维持的调节器:在这些细胞中上调，通过糖异生生成磷酸烯醇丙酮酸(通过相似性)。由此产生的磷酸烯醇丙酮酸流向糖原和戊糖磷酸通路，这对记忆CD8(+) t细胞的稳态至关重要(通过相似性)。除了磷酸烯醇丙酮酸羧激酶活性外，当Ser-90位点被磷酸化时，也作为蛋白激酶发挥作用:AKT1在Ser-90位点的磷酸化降低了与草酰乙酸的结合亲和力，并促进了使用GTP作为供体的非典型丝氨酸蛋白激酶活性(通过相似性)。蛋白激酶活性调节脂肪生成:在Ser-90位点磷酸化后，易位到内质网，并催化INSIG蛋白(INSIG1和INSIG2)的磷酸化，从而干扰INSIG蛋白与SCAP之间的相互作用，促进SREBP蛋白(SREBF1/SREBP1或SREBF2/SREBP2)的核易位和下游脂肪生成相关基因的后续转录(通过相似性)。磷酸烯醇丙酮酸羧激酶活性受乙酰化和葡萄糖水平的调节(PubMed:30193097)。 The anaplerotic conversion of phosphoenolpyruvate to oxaloacetate is improved by PCK1 acetylation on Lys-91 (K91ac), Lys-473 (K473ac) and Lys-521 (K521ac) (PubMed:30193097). High glucose concentrations favor PCK1 anaplerotic activity by triggering acetylation on Lys-91 (K91ac). At low glucose levels, SIRT1-mediated deacetylation of Lys-91 promotes the cataplerotic conversion of oxaloacetate to phosphoenolpyruvate (By similarity). Phosphoenolpyruvate carboxykinase activity is inhibited by 3-mercaptopicolinate (PubMed:26322521). Phosphoenolpyruvate carboxykinase activity is inhibited by 3-[(carboxymethyl)thio]picolinate (CMP), which acts as a competitive inhibitor at the oxaloacetate/phosphoenolpyruvate-binding site (PubMed:31461616). Phosphorylation at Ser-90 reduces the binding affinity to oxaloacetate and converts the enzyme into an atypical protein kinase using GTP as donor (By similarity).PATHWAY Carbohydrate biosynthesis; gluconeogenesis.SUBUNIT Monomer.INDUCTION Regulated by cAMP, dexamethasone, glucagon and by insulin. Dexamthasone, glucagon and cAMP increase levels, insulin decreases levels.PTM Acetylated (PubMed:30193097). Lysine acetylation by p300/EP300 is increased on high glucose conditions and promotes ubiquitination by UBR5; acetylation is enhanced in the presence of BAG6. Deacetylated by SIRT2. Deacetylation of Lys-91 is carried out by SIRT1 and depends on PCK1 phosphorylation levels (By similarity).PTM Phosphorylated in a GSK3B-mediated pathway; phosphorylation affects the efficiency of SIRT1-mediated deacetylation, and regulates PCK1 ubiquitination and degradation (PubMed:30193097). Phosphorylation at Ser-90 by AKT1 reduces the binding affinity to oxaloacetate and promotes the protein kinase activity: phosphorylated PCK1 translocates to the endoplasmic reticulum, where it phosphorylates INSIG1 and INSIG2 (By similarity).PTM Ubiquitination by UBR5 leads to proteasomal degradation.MISCELLANEOUS In eukaryotes there are two isozymes: a cytoplasmic one and a mitochondrial one.SIMILARITY Belongs to the phosphoenolpyruvate carboxykinase [GTP] family. 鼠形 NCBI分类法 10116 UniProt P07379 Reactome数据库ID Release 77 2161508 数据库标识符。使用此URL连接到Reactome中的此实例的网页://www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=2161508 Reactome r -优化- 2161508 1 Reactome稳定的标识符。使用此URL连接到Reactome中的此实例的网页://www.joaskin.com/cgi-bin/eventbrowser_st_id?ST_ID=R-RNO-2161508.1 基因本体论 去:0004550 细胞功能的基因本体论术语 心肌梗死 小姐:0355 相同的催化剂活性 Reactome数据库ID Release 77 2162076 数据库标识符。使用此URL连接到Reactome中的此实例的网页://www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=2162076 Reactome数据库ID Release 77 2162084 数据库标识符。使用此URL连接到Reactome中的此实例的网页://www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=2162084 Reactome r -优化- 2162084 2 Reactome稳定的标识符。使用此URL连接到Reactome中的此实例的网页://www.joaskin.com/cgi-bin/eventbrowser_st_id?ST_ID=R-RNO-2162084.2 1383219 Pubmed 1992 卡韦对映体的细胞酶磷酸化决定了抗病毒活性的立体选择性 米勒,WH Daluge, SM 加维,EP 霍普金斯,年代 里尔登,我 博伊德,FL 米勒,RL J Biol Chem 267:21220-4