BioPAX pathway converted from "Transport of Connexin to the Trans-Golgi network" in the Reactome database. LEFT-TO-RIGHT Transport of Connexin to the Trans-Golgi network Transport of connexins along the secretory pathway (including transit from the Golgi to the TGN where Cx43 is predicted to oligomerize) occurs in vesicular transport containers. Authored: Gilleron, J, Segretain, D, Falk, MM, 2007-01-03 12:23:09 Edited: Matthews, L, 2007-01-07 14:51:44 Cx43 Reactome DB_ID: 190688 endoplasmic reticulum membrane GENE ONTOLOGY 去:0005789 UniProt:P08050 Gja1 Gja1 Cxn-43 FUNCTION Gap junction protein that acts as a regulator of bladder capacity. A gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. Negative regulator of bladder functional capacity: acts by enhancing intercellular electrical and chemical transmission, thus sensitizing bladder muscles to cholinergic neural stimuli and causing them to contract (By similarity). May play a role in cell growth inhibition through the regulation of NOV expression and localization (PubMed:15181016). Plays an essential role in gap junction communication in the ventricles (By similarity).SUBUNIT A connexon is composed of a hexamer of connexins (PubMed:1371548). Interacts with SGSM3 (By similarity). Interacts with RIC1/CIP150 (By similarity). Interacts with CNST and CSNK1D (By similarity). Interacts (via C-terminus) with TJP1 (PubMed:15492000, PubMed:18636092). Interacts (via C-terminus) with SRC (via SH3 domain) (PubMed:15492000). Interacts (not ubiquitinated) with UBQLN4 (via UBA domain) (PubMed:20127391). Interacts with NOV (PubMed:15181016, PubMed:15213231). Interacts with TMEM65 (By similarity).TISSUE SPECIFICITY Detected in ventricle and atrium (at protein level).INDUCTION In bladder smooth muscle cells, exhibits night/day variations with low levels during the sleep phase, at circadian time (CT) 4-8 (at protein level). Expression starts to increase around CT12 and forms a plateau during the active phase (CT16-24) (at protein level).PTM Contains at least one intramolecular disulfide bond.PTM Phosphorylation at Ser-325, Ser-328 and Ser-330 by CK1 modulates gap junction assembly. Phosphorylated at Ser-368 by PRKCG; phosphorylation induces disassembly of gap junction plaques and inhibition of gap junction activity (By similarity). Phosphorylation at Ser-368 by PRKCD triggers its internalization into small vesicles leading to proteasome-mediated degradation (PubMed:24500718).PTM Sumoylated with SUMO1, SUMO2 and SUMO3, which may regulate the level of functional Cx43 gap junctions at the plasma membrane. May be desumoylated by SENP1 or SENP2 (By similarity).PTM S-nitrosylation at Cys-271 is enriched at the muscle endothelial gap junction in arteries, it augments channel permeability and may regulate of smooth muscle cell to endothelial cell communication.PTM Acetylated in the developing cortex; leading to delocalization from the cell membrane.SIMILARITY Belongs to the connexin family. Alpha-type (group II) subfamily. Rattus norvegicus NCBI Taxonomy 10116 UniProt P08050 Reactome Database ID Release 77 190688 Database identifier. Use this URL to connect to the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=190688 Reactome R-RNO-190688 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser_st_id?ST_ID=R-RNO-190688.1 Reactome //www.joaskin.com 6 Connexin 43 Reactome DB_ID: 190650 Golgi membrane GENE ONTOLOGY 去:0000139 Reactome Database ID Release 77 190650 Database identifier. Use this URL to connect to the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=190650 Reactome R-RNO-190650 1 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser_st_id?ST_ID=R-RNO-190650.1 6 Reactome Database ID Release 77 190685 Database identifier. Use this URL to connect to the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=190685 Reactome R-RNO-190685 2 Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser_st_id?ST_ID=R-RNO-190685.2 7691412 Pubmed 1993 Multisubunit assembly of an integral plasma membrane channel protein, gap junction connexin43, occurs after exit from the ER Musil, LS 前言,哒 Cell 74:1065-77