BioPAX pathway converted from "Synthesis of active ubiquitin: roles of E1 and E2 enzymes" in the Reactome database. Synthesis of active ubiquitin: roles of E1 and E2 enzymes Synthesis of active ubiquitin: roles of E1 and E2 enzymes Ubiquitin monomers are processed from larger precursors and then activated by formation of a thiol ester bond between ubiquitin and a cysteine residue of an E1 activating enzyme (UBA1 or UBA6, Jin et al. 2007). The ubiquitin is then transferred to the active site cysteine residue of an E2 conjugating enzyme (reviewed in van Wijk and Timmers 2010, Kleiger and Mayor 2014, Stewart et al. 2016). Precursor proteins containing multiple ubiquitin monomers (polyubiquitins) are produced from the UBB and UBC genes. Precursors containing a single ubiquitin fused to a ribosomal protein are produced from the UBA52 and RPS27A genes. The proteases OTULIN and USP5 are very active in polyubiquitin processing, whereas the proteases UCHL3, USP7, and USP9X cleave the ubiquitin-ribosomal protein precursors yielding ubiquitin monomers (Grou et al. 2015). Other enzymes may also process ubiquitin precursors. A resultant ubiquitin monomer is activated by adenylation of its C-terminal glycine followed by conjugation of the C-terminus to a cysteine residue of the E1 enzymes UBA1 or UBA6 via a thiol ester bond (Jin et al. 2007, inferred from rabbit homologues in Haas et al. 1982, Hershko et al. 1983). The ubiquitin is then transferred from the E1 enzyme to a cysteine residue of one of several E2 enzymes (reviewed in van Wijk and Timmers 2010, Stewart et al. 2016). Authored: May, Bruce, 2016-04-02 Reviewed: Azevedo, Jorge E, 2016-08-11 Edited: May, Bruce, 2016-04-02 3.4.19.12 OTULIN,USP5 cleaves UBB yielding ubiquitin OTULIN,USP5 cleaves UBB yielding ubiquitin The UBB poly-protein contains 3 Ubiquitin monomers that are released by proteolysis. Either USP5 (Falquet et al. 1995) or OTULIN can perform the proteolyic cleavage reactions (Grou et al. 2015). Authored: May, Bruce, 2016-01-14 Reviewed: Azevedo, Jorge E, 2016-08-11 Edited: May, Bruce, 2016-01-14 Reactome DB_ID: 8853516 1 cytosol GO 0005829 UniProt:P0CG47 UBB UBB UBB SUBUNIT Interacts with SKP1-KMD2A and SKP1-KMD2B complexes.MISCELLANEOUS Ubiquitin is encoded by 4 different genes. UBA52 and RPS27A genes code for a single copy of ubiquitin fused to the ribosomal proteins L40 and S27a, respectively. UBB and UBC genes code for a polyubiquitin precursor with exact head to tail repeats, the number of repeats differ between species and strains.MISCELLANEOUS The mRNA encoding variant UBB(+1) is produced by an unknown mechanism involving the deletion of a GT dinucleotide in the close proximity of a GAGAG motif (PubMed:9422699). This variant mRNA is found in normal brain, but the encoded protein accumulates only in brain neurofibrillary tangles and neuritic plaques in Alzheimer disease and other tauopathies, as well as polyglutaminopathies (PubMed:14597671). UBB(+1) variant cannot be used for polyubiquitination, is not effectively degraded by the proteasome when ubiquitinated and ubiquitinated UBB(+1) is refractory to disassembly by deubiquitinating enzymes (DUBs). In healthy brain, UBB(+1) C-terminus can be cleaved by UCHL3 (PubMed:21762696).MISCELLANEOUS For a better understanding, features related to ubiquitin are only indicated for the first chain.SIMILARITY Belongs to the ubiquitin family. Reactome //www.joaskin.com Homo sapiens NCBI分类法 9606 UniProt P0CG47 Chain Coordinates 1 EQUAL 229 EQUAL Reactome DB_ID: 939230 1 153 EQUAL 228 EQUAL Reactome DB_ID: 939213 1 77 EQUAL 152 EQUAL Reactome DB_ID: 939214 1 1 EQUAL 76 EQUAL PHYSIOL-LEFT-TO-RIGHT ACTIVATION Converted from EntitySet in Reactome Reactome DB_ID: 8853527 OTULIN,USP5 [cytosol] Converted from EntitySet in Reactome. Each synonym is a name of a PhysicalEntity, and each XREF points to one PhysicalEntity USP5 [cytosol] OTULIN [cytosol] UniProt P45974 UniProt Q96BN8 GO 0004843 GO molecular function Reactome Database ID Release 77 8853512 数据库标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=8853512 Reactome Database ID Release 77 8853529 数据库标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=8853529 Reactome R-HSA-8853529 2 Reactome稳定的标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser_st_id?ST_ID=R-HSA-8853529.2 26235645 Pubmed 2015 泛素的从头合成:识别of deubiquitinases acting on ubiquitin precursors Grou, Cláudia P Pinto, Manuel P Mendes, Andreia V Domingues, Pedro Azevedo, Jorge E Sci Rep 5:12836 7851534 Pubmed 1995 A human de-ubiquitinating enzyme with both isopeptidase and peptidase activities in vitro Falquet, L Paquet, N Frutiger, S Hughes, G J Hoang-Van, K Jaton, J C FEBS Lett. 359:73-7 3.4.19.12 OTULIN,USP5 cleaves UBC yielding ubiquitin OTULIN,USP5 cleaves UBC yielding ubiquitin The UBC poly-protein contains 9 Ubiquitin monomers that are released by proteolysis. Either USP5 (Falquet et al. 1995) or OTULIN can perform the proteolyic cleavage reactions (Grou et al. 2015). Authored: May, Bruce, 2016-01-14 Reviewed: Azevedo, Jorge E, 2016-08-11 Edited: May, Bruce, 2016-01-14 Reactome DB_ID: 8853531 1 UniProt:P0CG48 UBC UBC UBC MISCELLANEOUS Ubiquitin is encoded by 4 different genes. UBA52 and RPS27A genes code for a single copy of ubiquitin fused to the ribosomal proteins L40 and S27a, respectively. UBB and UBC genes code for a polyubiquitin precursor with exact head to tail repeats, the number of repeats differ between species and strains.MISCELLANEOUS For the sake of clarity sequence features are annotated only for the first chain, and are not repeated for each of the following chains.SIMILARITY Belongs to the ubiquitin family. UniProt P0CG48 1 EQUAL 685 EQUAL Reactome DB_ID: 939192 1 229 EQUAL 304 EQUAL Reactome DB_ID: 939188 1 1 EQUAL 76 EQUAL Reactome DB_ID: 939165 1 609 EQUAL 684 EQUAL Reactome DB_ID: 939184 1 457 EQUAL 532 EQUAL Reactome DB_ID: 939232 1 305 EQUAL 380 EQUAL Reactome DB_ID: 939164 1 77 EQUAL 152 EQUAL Reactome DB_ID: 939258 1 153 EQUAL 228 EQUAL Reactome DB_ID: 939191 1 381 EQUAL 456 EQUAL Reactome DB_ID: 939239 1 533 EQUAL 608 EQUAL PHYSIOL-LEFT-TO-RIGHT ACTIVATION Converted from EntitySet in Reactome Reactome DB_ID: 8853527 Reactome Database ID Release 77 8853515 数据库标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=8853515 Reactome R-HSA-8853515 2 Reactome稳定的标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser_st_id?ST_ID=R-HSA-8853515.2 3.4.19.12 UCHL3,USP7,USP9X cleaves UBA52 yielding ubiquitin UCHL3,USP7,USP9X cleaves UBA52 yielding ubiquitin The UBA52 precursor protein contains the ribosomal protein L40 and ubiquitin (UBA52 residues 1 to 76) which are released by proteolysis. Any of the proteases UCHL3, USP7, or USP9X can catalyze the proteolysis reaction (Larsen et al. 1998, Grou et al. 2015). Authored: May, Bruce, 2016-01-14 Reviewed: Azevedo, Jorge E, 2016-08-11 Edited: May, Bruce, 2016-01-14 Reactome DB_ID: 8853517 1 UniProt:P62987 UBA52 UBA52 UBCEP2 UBA52 SUBUNIT Ribosomal protein L40 is part of the 60S ribosomal subunit. Interacts with UBQLN1 (via UBA domain).MISCELLANEOUS Ubiquitin is encoded by 4 different genes. UBA52 and RPS27A genes code for a single copy of ubiquitin fused to the ribosomal proteins L40 and S27a, respectively. UBB and UBC genes code for a polyubiquitin precursor with exact head to tail repeats, the number of repeats differ between species and strains.MISCELLANEOUS For a better understanding, features related to ubiquitin are only indicated for the first chain.SIMILARITY In the N-terminal section; belongs to the ubiquitin family.SIMILARITY In the C-terminal section; belongs to the eukaryotic ribosomal protein eL40 family. UniProt P62987 1 EQUAL 128 EQUAL Reactome DB_ID: 939203 1 1 EQUAL 76 EQUAL Reactome DB_ID: 72476 1 77 EQUAL 128 EQUAL PHYSIOL-LEFT-TO-RIGHT ACTIVATION Converted from EntitySet in Reactome Reactome DB_ID: 8853523 UCHL3,USP7,USP9X [cytosol] Converted from EntitySet in Reactome. Each synonym is a name of a PhysicalEntity, and each XREF points to one PhysicalEntity USP9X [cytosol] UCHL3 [cytosol] USP7 [cytosol] UniProt Q93008 UniProt P15374 UniProt Q93009 Reactome Database ID Release 77 8853522 数据库标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=8853522 Reactome Database ID Release 77 8853514 数据库标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=8853514 Reactome R-HSA-8853514 2 Reactome稳定的标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser_st_id?ST_ID=R-HSA-8853514.2 9521656 Pubmed 1998 Substrate specificity of deubiquitinating enzymes: ubiquitin C-terminal hydrolases Larsen, C N Krantz, B A Wilkinson, K D Biochemistry 37:3358-68 3.4.19.12 UCHL3,USP7,USP9X cleaves RPS27A yielding ubiquitin UCHL3,USP7,USP9X cleaves RPS27A yielding ubiquitin The RPS27A precursor protein contains the ribosomal protein L40 and ubiquitin (RPS27A residues 1 to 76) which are released by proteolysis. Any of the proteases UCHL3, USP7, or USP9X can catalyze the proteolysis reaction (Larsen et al. 1998, Grou et al. 2015). Authored: May, Bruce, 2016-01-14 Reviewed: Azevedo, Jorge E, 2016-08-11 Edited: May, Bruce, 2016-01-14 Reactome DB_ID: 8853524 1 UniProt:P62979 RPS27A RPS27A UBA80 RPS27A UBCEP1 亚基核糖体蛋白S27a是40年代的一部分ribosomal subunit.MISCELLANEOUS Ubiquitin is encoded by 4 different genes. UBA52 and RPS27A genes code for a single copy of ubiquitin fused to the ribosomal proteins L40 and S27a, respectively. UBB and UBC genes code for a polyubiquitin precursor with exact head to tail repeats, the number of repeats differ between species and strains.MISCELLANEOUS For a better understanding, features related to ubiquitin are only indicated for the first chain.SIMILARITY In the N-terminal section; belongs to the ubiquitin family.SIMILARITY In the C-terminal section; belongs to the eukaryotic ribosomal protein eS31 family. UniProt P62979 1 EQUAL 76 EQUAL Reactome DB_ID: 939205 1 1 EQUAL 76 EQUAL Reactome DB_ID: 72363 1 77 EQUAL 156 EQUAL PHYSIOL-LEFT-TO-RIGHT ACTIVATION Converted from EntitySet in Reactome Reactome DB_ID: 8853523 Reactome Database ID Release 77 8853503 数据库标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=8853503 Reactome R-HSA-8853503 2 Reactome稳定的标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser_st_id?ST_ID=R-HSA-8853503.2 6.2.1.45 UBA1 adenylates ubiquitin in the cytosol UBA1 adenylates ubiquitin in the cytosol Activation of ubiquitin by UBA1 proceeds through 3 steps: adenylation of ubiquitin, conjugation of ubiquitin from adenyl-ubiquitin to an internal cysteine residue of UBA1, and adenylation of a second molecule of ubiquitin. In the first step the adenylation module of UBA1 catalyzes the acyl-adenylation of the C-terminal glycine of ubiquitin (Jin et al. 2007, inferred from the rabbit homologue in Haas et al. 1982, Hershko et al. 1983, reviewed in Groettrup et al. 2008). Authored: May, Bruce, 2016-01-11 Reviewed: Azevedo, Jorge E, 2016-08-11 Edited: May, Bruce, 2016-01-11 Reactome DB_ID: 947698 1 UniProt:P22314 UBA1 UBA1 A1S9T UBA1 UBE1 FUNCTION Catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation through the ubiquitin-proteasome system (PubMed:1606621, PubMed:1447181). Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a ubiquitin-E1 thioester and free AMP (PubMed:1447181). Essential for the formation of radiation-induced foci, timely DNA repair and for response to replication stress. Promotes the recruitment of TP53BP1 and BRCA1 at DNA damage sites (PubMed:22456334).PATHWAY Protein modification; protein ubiquitination.SUBUNIT Monomer (By similarity). Interacts with GAN (via BTB domain).TISSUE SPECIFICITY Detected in erythrocytes (at protein level). Ubiquitous.DOMAIN The first 11 amino acids are essential for phosphorylation and exclusive nuclear localization.PTM ISGylated.MISCELLANEOUS There are two active sites within the E1 molecule, allowing it to accommodate two ubiquitin moieties at a time, with a new ubiquitin forming an adenylate intermediate as the previous one is transferred to the thiol site.SIMILARITY Belongs to the ubiquitin-activating E1 family. UniProt P22314 41 EQUAL 1058 EQUAL Converted from EntitySet in Reactome Reactome DB_ID: 113595 1 Ub [cytosol] Converted from EntitySet in Reactome. Each synonym is a name of a PhysicalEntity, and each XREF points to one PhysicalEntity UBC(229-304) [cytosol] UBB(77-152) [cytosol] UBA52(1-76) [cytosol] UBC(1-76) [cytosol] UBC(77-152) [cytosol] UBC(381-456) [cytosol] UBC(533-608) [cytosol] UBB(153-228) [cytosol] RPS27A(1-76) [cytosol] UBB(1-76) [cytosol] UBC(609-684) [cytosol] UBC(457-532) [cytosol] UBC(305-380) [cytosol] UBC(153-228) [cytosol] Reactome DB_ID: 113592 1 ATP(4-) [ChEBI:30616] ATP(4-) Adenosine 5'-triphosphate atp ATP ChEBI 30616 Reactome DB_ID: 8852068 1 UBA1:泛素腺苷酸[cytosol] UBA1:泛素腺苷酸 Converted from EntitySet in Reactome Reactome DB_ID: 8852080 1 ubiquitin adenylate [cytosol] Converted from EntitySet in Reactome. Each synonym is a name of a PhysicalEntity, and each XREF points to one PhysicalEntity adenylatedG684-UBC(609-684) [cytosol] adenylatedG304-UBC(229-304) [cytosol] adenylatedG152-UBC(77-152) [cytosol] adenylatedG608-UBC(533-608) [cytosol] adenylatedG76-UBC(1-76) [cytosol] adenylatedG228-UBC(153-228) [cytosol] adenylatedG152-UBB(77-152) [cytosol] adenylatedG76-UBA52(1-76) [cytosol] adenylatedlG76-RPS27A(1-76) [cytosol] adenylatedG456-UBC(381-456) [cytosol] adenylatedG76-UBB(1-76) [cytosol] adenylatedG532-UBC(457-532) [cytosol] adenylatedG380-UBC(305-380) [cytosol] adenylatedG228-UBB(153-228) [cytosol] Reactome DB_ID: 947698 1 41 EQUAL 1058 EQUAL Reactome Database ID Release 77 8852068 数据库标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=8852068 Reactome R-HSA-8852068 1 Reactome稳定的标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser_st_id?ST_ID=R-HSA-8852068.1 Reactome DB_ID: 111294 1 diphosphoric acid [ChEBI:29888] diphosphoric acid ChEBI 29888 PHYSIOL-LEFT-TO-RIGHT ACTIVATION Reactome DB_ID: 947698 41 EQUAL 1058 EQUAL GO 0004839 GO molecular function Reactome Database ID Release 77 8865063 数据库标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=8865063 Reactome Database ID Release 77 8852134 数据库标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=8852134 Reactome R-HSA-8852134 1 Reactome稳定的标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser_st_id?ST_ID=R-HSA-8852134.1 17597759 Pubmed 2007 Dual E1 activation systems for ubiquitin differentially regulate E2 enzyme charging Jin, J Li, Xue Gygi, SP Harper, J Wade Nature 447:1135-8 18353650 Pubmed 2008 Activating the ubiquitin family: UBA6 challenges the field Groettrup, Marcus Pelzer, Christiane Schmidtke, Gunter Hofmann, Kay Trends Biochem. Sci. 33:230-7 6305978 Pubmed 1983 Components of ubiquitin-protein ligase system. Resolution, affinity purification, and role in protein breakdown Hershko, A Heller, H Elias, S Ciechanover, A J. Biol. Chem. 258:8206-14 6277905 Pubmed 1982 Ubiquitin-activating enzyme. Mechanism and role in protein-ubiquitin conjugation Haas, A L Warms, J V Hershko, A Rose, I A J. Biol. Chem. 257:2543-8 6.2.1.45 UBA1 conjugates ubiquitin to UBA1 in the cytosol UBA1 conjugates ubiquitin to UBA1 in the cytosol Activation of ubiquitin by UBA1 proceeds through 3 steps: adenylation of ubiquitin, conjugation of ubiquitin from adenyl-ubiquitin to an internal cysteine residue of UBA1, and adenylation of a second molecule of ubiquitin. In the second step, the thiol group of an internal cysteine residue of UBA1 attacks the acyl-adenyl bond of the C-terminal glycine of adenylated ubiquitin, resulting in a thioester bond between the cysteine residue and the C-terminal glycine of ubiquitin (Jin et al. 2007, inferred from the rabbit homologue in Haas et al. 1982, Hershko et al. 1983, reviewed in Groettrup et al. 2008). Authored: May, Bruce, 2016-01-11 Reviewed: Azevedo, Jorge E, 2016-08-11 Edited: May, Bruce, 2016-01-11 Reactome DB_ID: 8852068 1 Reactome DB_ID: 8852063 1 Ub-Cys632-UBA1 [cytosol] Ub-Cys632-UBA1 Reactome DB_ID: 947698 1 41 EQUAL 1058 EQUAL Converted from EntitySet in Reactome Reactome DB_ID: 113595 1 Reactome Database ID Release 77 8852063 数据库标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=8852063 Reactome R-HSA-8852063 1 Reactome稳定的标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser_st_id?ST_ID=R-HSA-8852063.1 Reactome DB_ID: 76577 1 adenosine 5'-monophosphate [ChEBI:16027] adenosine 5'-monophosphate ChEBI 16027 PHYSIOL-LEFT-TO-RIGHT ACTIVATION Reactome DB_ID: 8852068 Reactome Database ID Release 77 8865083 数据库标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=8865083 Reactome Database ID Release 77 8852133 数据库标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=8852133 Reactome R-HSA-8852133 1 Reactome稳定的标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser_st_id?ST_ID=R-HSA-8852133.1 17580310 Pubmed 2007 UBE1L2, a novel E1 enzyme specific for ubiquitin Pelzer, Christiane Kassner, Ingrid Matentzoglu, Konstantin Singh, Rajesh K Wollscheid, Hans-Peter Scheffner, Martin Schmidtke, Gunter Groettrup, Marcus J. Biol. Chem. 282:23010-4 6.2.1.45 Ub-Cys632-UBA1 adenylates ubiquitin in the cytosol Ub-Cys632-UBA1 adenylates ubiquitin in the cytosol Activation of ubiquitin by UBA1 proceeds through 3 steps: adenylation of ubiquitin, conjugation of ubiquitin from adenyl-ubiquitin to an internal cysteine residue of UBA1, and adenylation of a second molecule of ubiquitin. In the third step, the UBA1-ubiquitin conjugate adenylates the C-terminal glycine of a second ubiquitin molecule. This results in UBA1 loaded with 2 ubiquitin molecules, one of which is conjugated to an internal cysteine residue of UBA1 and one of which is adenylated and non-covalently bound to UBA1 (Jin et al. 2007, inferred from the rabbit homologue in Haas et al. 1982, Hershko et al. 1983, reviewed in Groettrup et al. 2008). Authored: May, Bruce, 2016-01-11 Reviewed: Azevedo, Jorge E, 2016-08-11 Edited: May, Bruce, 2016-01-11 Reactome DB_ID: 8852063 1 Converted from EntitySet in Reactome Reactome DB_ID: 113595 1 Reactome DB_ID: 113592 1 Reactome DB_ID: 8852079 1 Ub-Cys632-UBA1:泛素亚丁湾ylate [cytosol] Ub-Cys632-UBA1:泛素亚丁湾ylate Converted from EntitySet in Reactome Reactome DB_ID: 8852080 1 Reactome DB_ID: 8852063 1 Reactome Database ID Release 77 8852079 数据库标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=8852079 Reactome R-HSA-8852079 1 Reactome稳定的标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser_st_id?ST_ID=R-HSA-8852079.1 Reactome DB_ID: 111294 1 PHYSIOL-LEFT-TO-RIGHT ACTIVATION Reactome DB_ID: 8852063 Reactome Database ID Release 77 8865054 数据库标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=8865054 Reactome Database ID Release 77 8852132 数据库标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=8852132 Reactome R-HSA-8852132 1 Reactome稳定的标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser_st_id?ST_ID=R-HSA-8852132.1 UBA1 conjugates ubiquitin to cytosolic E2 enzymes UBA1 conjugates ubiquitin to cytosolic E2 enzymes In the cytosol, the UBA1-ubiquitin thiol ester conjugate transfers ubiquitin from UBA1 to an internal cysteine residue of the E2 enzyme, forming a thiol ester conjugate between ubiquitin and the cysteine residue of E2 (Jin et al. 2007, inferred from the rabbit homologue in Hershko et al. 1983). The E2 then disengages from UBA1 (Eletr et al. 2005). Authored: May, Bruce, 2016-01-11 Reviewed: Azevedo, Jorge E, 2016-08-11 Edited: May, Bruce, 2016-01-11 Reactome DB_ID: 8852079 1 Converted from EntitySet in Reactome Reactome DB_ID: 8852103 1 E2 ubiquitin conjugating enzyme (UBA1 substrate) [cytosol] Converted from EntitySet in Reactome. Each synonym is a name of a PhysicalEntity, and each XREF points to one PhysicalEntity UBE2S [cytosol] UBE2D1(胞质) UBE2Q2 [cytosol] UBE2K [cytosol] UBE2R2 [cytosol] UBE2L3 [cytosol] UBE2G2 [cytosol] UBE2E3 [cytosol] UBE2G1 [cytosol] UBE2C [cytosol] UBE2D2 [cytosol] CDC34 [cytosol] UBE2H [cytosol] UniProt Q16763 UniProt P51668 UniProt Q8WVN8 UniProt P61086 UniProt Q712K3 UniProt P68036 UniProt P60604 UniProt Q969T4 UniProt P62253 UniProt O00762 UniProt P62837 UniProt P49427 UniProt P62256 Reactome DB_ID: 8852251 1 Ub:E2 (UBA1 substrate) [cytosol] Ub:E2 (UBA1 substrate) Converted from EntitySet in Reactome Reactome DB_ID: 8864150 1 Ub-conjugated E2 enzyme (UBA1 substrate) [cytosol] Converted from EntitySet in Reactome. Each synonym is a name of a PhysicalEntity, and each XREF points to one PhysicalEntity Ub-C92-UBE2K [cytosol] Ub-C86-UBE2L3 [cytosol] Ub-C87-UBE2H [cytosol] Ub-C93-CDC34 [cytosol] Ub-C85-UBE2D2 [cytosol] Ub-C114-UBE2C [cytosol] Ub-C90-UBE2G1 [cytosol] Ub-C93-UBE2R2 [cytosol] Ub-C89-UBE2G2 [cytosol] Ub-C95-UBE2S [cytosol] Ub-C304-UBE2Q2 [cytosol] Ub-C145-UBE2E3 [cytosol] Ub-C85-UBE2D1 [cytosol] Converted from EntitySet in Reactome Reactome DB_ID: 113595 1 Reactome Database ID Release 77 8852251 数据库标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=8852251 Reactome R-HSA-8852251 1 Reactome稳定的标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser_st_id?ST_ID=R-HSA-8852251.1 Reactome DB_ID: 8852068 1 Reactome Database ID Release 77 8852129 数据库标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=8852129 Reactome R-HSA-8852129 1 Reactome稳定的标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser_st_id?ST_ID=R-HSA-8852129.1 16142244 Pubmed 2005 E2 conjugating enzymes must disengage from their E1 enzymes before E3-dependent ubiquitin and ubiquitin-like transfer Eletr, Ziad M Huang, Danny T Duda, David M Schulman, Brenda A Kuhlman, Brian Nat. Struct. Mol. Biol. 12:933-4 6.2.1.45 UBA6 adenylates ubiquitin in the cytosol UBA6 adenylates ubiquitin in the cytosol 从相应的UBA1推断,激活啊f ubiquitin by UBA6 (UBE1L1) proceeds through 3 steps: adenylation of ubiquitin, conjugation of ubiquitin from adenyl-ubiquitin to an internal cysteine residue of UBA6, and adenylation of a second molecule of ubiquitin. In the first step, the adenylation module of UBA6 catalyzes the acyl-adenylation of the C-terminal glycine of ubiquitin (Jin et al. 2007, Pelzer et al. 2007, Groettrup et al. 2008). Authored: May, Bruce, 2016-01-11 Reviewed: Azevedo, Jorge E, 2016-08-11 Edited: May, Bruce, 2016-01-11 Converted from EntitySet in Reactome Reactome DB_ID: 113595 1 Reactome DB_ID: 113592 1 Reactome DB_ID: 947701 1 UniProt:A0AVT1 UBA6 UBA6 UBE1L2 MOP4 UBA6 FUNCTION Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a ubiquitin-E1 thioester and free AMP. Specific for ubiquitin, does not activate ubiquitin-like peptides. Differs from UBE1 in its specificity for substrate E2 charging. Does not charge cell cycle E2s, such as CDC34. Essential for embryonic development. Required for UBD/FAT10 conjugation. Isoform 2 may play a key role in ubiquitin system and may influence spermatogenesis and male fertility.PATHWAY Protein modification; protein ubiquitination.SUBUNIT Forms a thioester with UBD in cells stimulated with tumor necrosis factor-alpha (TNFa) and interferon-gamma (IFNg) (PubMed:17889673, PubMed:25422469).TISSUE SPECIFICITY Widely expressed. Isoform 2 is predominantly expressed in testis with higher expression in adult testis than in fetal testis.SIMILARITY Belongs to the ubiquitin-activating E1 family. UniProt A0AVT1 1 EQUAL 1052 EQUAL Reactome DB_ID: 8852088 1 UBA6:ubiquitin adenylate [cytosol] UBA6:ubiquitin adenylate Converted from EntitySet in Reactome Reactome DB_ID: 8852080 1 Reactome DB_ID: 947701 1 1 EQUAL 1052 EQUAL Reactome Database ID Release 77 8852088 数据库标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=8852088 Reactome R-HSA-8852088 1 Reactome稳定的标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser_st_id?ST_ID=R-HSA-8852088.1 Reactome DB_ID: 111294 1 PHYSIOL-LEFT-TO-RIGHT ACTIVATION Reactome DB_ID: 947701 1 EQUAL 1052 EQUAL Reactome Database ID Release 77 8865189 数据库标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=8865189 Reactome Database ID Release 77 8865090 数据库标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=8865090 Reactome R-HSA-8865090 1 Reactome稳定的标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser_st_id?ST_ID=R-HSA-8865090.1 6.2.1.45 UBA6 conjugates ubiquitin to UBA6 in the cytosol UBA6 conjugates ubiquitin to UBA6 in the cytosol 从相应的UBA1推断,激活啊f ubiquitin by UBA6 (UBE1L1) proceeds through 3 steps: adenylation of ubiquitin, conjugation of ubiquitin from adenyl-ubiquitin to an internal cysteine residue of UBA6, and adenylation of a second molecule of ubiquitin. In the second step, the thiol group of an internal cysteine residue of UBA6 attacks the acyl-adenyl bond of the C-terminal glycine of adenylated ubiquitin, resulting in a thioester bond between the cysteine residue and the C-terminal glycine of ubiquitin (Jin et al. 2007, Pelzer et al. 2007, reviewed in Groettrup et al. 2008). Authored: May, Bruce, 2016-01-11 Reviewed: Azevedo, Jorge E, 2016-08-11 Edited: May, Bruce, 2016-01-11 Reactome DB_ID: 8852088 1 Reactome DB_ID: 8852066 1 Ub-Cys625-UBA6 [cytosol] Ub-Cys625-UBA6 Converted from EntitySet in Reactome Reactome DB_ID: 113595 1 Reactome DB_ID: 947701 1 1 EQUAL 1052 EQUAL Reactome Database ID Release 77 8852066 数据库标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=8852066 Reactome R-HSA-8852066 1 Reactome稳定的标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser_st_id?ST_ID=R-HSA-8852066.1 Reactome DB_ID: 76577 1 PHYSIOL-LEFT-TO-RIGHT ACTIVATION Reactome DB_ID: 8852088 Reactome Database ID Release 77 8865076 数据库标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=8865076 Reactome Database ID Release 77 8865098 数据库标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=8865098 Reactome R-HSA-8865098 2 Reactome稳定的标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser_st_id?ST_ID=R-HSA-8865098.2 6.2.1.45 Ub-Cys625-UBA6 adenylates ubiquitin in the cytosol Ub-Cys625-UBA6 adenylates ubiquitin in the cytosol 从相应的UBA1推断,激活啊f ubiquitin by UBA6 (UBE1L1) proceeds through 3 steps: adenylation of ubiquitin, conjugation of ubiquitin from adenyl-ubiquitin to an internal cysteine residue of UBA6, and adenylation of a second molecule of ubiquitin. In the third step, the UBA6-ubiquitin conjugate adenylates the C-terminal glycine of a second ubiquitin molecule. This results in UBA6 loaded with 2 ubiquitin molecules, one of which is conjugated to an internal cysteine residue of UBA6 and one of which is adenylated and non-covalently bound to UBA6 (Jin et al. 2007, Pelzer et al. 2007, reviewed in Groettrup et al. 2008). Authored: May, Bruce, 2016-01-11 Reviewed: Azevedo, Jorge E, 2016-08-11 Edited: May, Bruce, 2016-01-11 Reactome DB_ID: 8852066 1 Converted from EntitySet in Reactome Reactome DB_ID: 113595 1 Reactome DB_ID: 113592 1 Reactome DB_ID: 111294 1 Reactome DB_ID: 8852097 1 Ub-Cys625-UBA6:ubiquitin adenylate [cytosol] Ub-Cys625-UBA6:ubiquitin adenylate Converted from EntitySet in Reactome Reactome DB_ID: 8852080 1 Reactome DB_ID: 8852066 1 Reactome Database ID Release 77 8852097 数据库标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=8852097 Reactome R-HSA-8852097 1 Reactome稳定的标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser_st_id?ST_ID=R-HSA-8852097.1 PHYSIOL-LEFT-TO-RIGHT ACTIVATION Reactome DB_ID: 8852066 Reactome Database ID Release 77 8865193 数据库标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=8865193 Reactome Database ID Release 77 8865050 数据库标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=8865050 Reactome R-HSA-8865050 1 Reactome稳定的标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser_st_id?ST_ID=R-HSA-8865050.1 UBA6 conjugates ubiquitin to cytosolic E2 enzymes UBA6 conjugates ubiquitin to cytosolic E2 enzymes In the cytosol, the UBA6-ubiquitin thiol ester conjugate transfers ubiquitin from UBA6 to an internal cysteine residue of the E2 enzyme, forming a thiol ester conjugate between ubiquitin and the cysteine residue of E2 (Jin et al. 2007, inferred from the rabbit homologue in Hershko et al. 1983, reviewed in Groettrup et al. 2008). As inferred from UBA1, the E2 then disengages from UBA6 (Eletr et al. 2005). Authored: May, Bruce, 2016-01-11 Reviewed: Azevedo, Jorge E, 2016-08-11 Edited: May, Bruce, 2016-01-11 Converted from EntitySet in Reactome Reactome DB_ID: 8852061 1 E2 ubiquitin conjugating enzyme (UBA6 substrate) [cytosol] Converted from EntitySet in Reactome. Each synonym is a name of a PhysicalEntity, and each XREF points to one PhysicalEntity UBE2L3 [cytosol] UBE2G2 [cytosol] UBE2E3 [cytosol] UBE2Z [cytosol] UBE2S [cytosol] UBE2D1(胞质) UBE2D2 [cytosol] UniProt Q9H832 Reactome DB_ID: 8852097 1 Reactome DB_ID: 8864229 1 Ub:E2 (UBA6 substrate) [cytosol] Ub:E2 (UBA6 substrate) Converted from EntitySet in Reactome Reactome DB_ID: 113595 1 Converted from EntitySet in Reactome Reactome DB_ID: 8864192 1 Ub-conjugated E2 enzyme (UBA6 substrate) [cytosol] Converted from EntitySet in Reactome. Each synonym is a name of a PhysicalEntity, and each XREF points to one PhysicalEntity Ub-C188-UBE2Z [cytosol] Ub-C89-UBE2G2 [cytosol] Ub-C95-UBE2S [cytosol] Ub-C86-UBE2L3 [cytosol] Ub-C145-UBE2E3 [cytosol] Ub-C85-UBE2D1 [cytosol] Ub-C85-UBE2D2 [cytosol] Reactome Database ID Release 77 8864229 数据库标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=8864229 Reactome R-HSA-8864229 1 Reactome稳定的标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser_st_id?ST_ID=R-HSA-8864229.1 Reactome DB_ID: 8852088 1 Reactome Database ID Release 77 8852127 数据库标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=8852127 Reactome R-HSA-8852127 1 Reactome稳定的标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser_st_id?ST_ID=R-HSA-8852127.1 UBA1 adenylates ubiquitin in the nucleoplasm UBA1 adenylates ubiquitin in the nucleoplasm UBA1 is present in both the cytosol and nucleoplasm (Grenfell et al. 1994). Activation of ubiquitin by UBA1 proceeds through 3 steps: adenylation of ubiquitin, conjugation of ubiquitin from adenyl-ubiquitin to an internal cysteine residue of UBA1, and adenylation of a second molecule of ubiquitin. In the first step, the adenylation module of UBA1 catalyzes the acyl-adenylation of the C-terminal glycine of ubiquitin (Jin et al. 2007, inferred from the rabbit homologue in Haas et al. 1982, Hershko et al. 1983, reviewed in Groettrup et al. 2008). Authored: May, Bruce, 2016-01-11 Reviewed: Azevedo, Jorge E, 2016-08-11 Edited: May, Bruce, 2016-01-11 Reactome DB_ID: 8852116 1 nucleoplasm GO 0005654 2 EQUAL 1058 EQUAL Reactome DB_ID: 29358 1 Converted from EntitySet in Reactome Reactome DB_ID: 68524 1 Ub [nucleoplasm] Converted from EntitySet in Reactome. Each synonym is a name of a PhysicalEntity, and each XREF points to one PhysicalEntity UBC(533-608) [nucleoplasm] UBC(305-380) [nucleoplasm] UBC(229-304) [nucleoplasm] UBC(457-532) [nucleoplasm] UBC(1-76) [nucleoplasm] UBA52(1-76) [nucleoplasm] UBC(153-228) [nucleoplasm] UBB(1-76) [nucleoplasm] UBC(381-456) [nucleoplasm] UBC(609-684) [nucleoplasm] UBB(77-152) [nucleoplasm] RPS27A(1-76) [nucleoplasm] UBB(153-228) [nucleoplasm] UBC(77-152) [nucleoplasm] Reactome DB_ID: 8852055 1 UBA1:泛素腺苷酸[nucleoplasm] UBA1:泛素腺苷酸 Reactome DB_ID: 8852116 1 2 EQUAL 1058 EQUAL Converted from EntitySet in Reactome Reactome DB_ID: 8852106 1 ubiquitin adenylate [nucleoplasm] Converted from EntitySet in Reactome. Each synonym is a name of a PhysicalEntity, and each XREF points to one PhysicalEntity adenylatedG152-UBB(77-152) [nucleoplasm] adenylatedG228-UBB(153-228) [nucleoplasm] adenylatedG456-UBC(381-456) [nucleoplasm] adenylatedG684-UBC(609-684) [nucleoplasm] adenylatedG608-UBC(533-608) [nucleoplasm] adenylatedG380-UBC(305-380) [nucleoplasm] adenylatedG76-UBB(1-76) [nucleoplasm] adenylatedlG76-RPS27A(1-76) [nucleoplasm] adenylatedG76-UBC(1-76) [nucleoplasm] adenylatedG304-UBC(229-304) [nucleoplasm] adenylatedG228-UBC(153-228) [nucleoplasm] adenylatedG152-UBC(77-152) [nucleoplasm] adenylatedG532-UBC(457-532) [nucleoplasm] adenylatedG76-UBA52(1-76) [nucleoplasm] Reactome Database ID Release 77 8852055 数据库标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=8852055 Reactome R-HSA-8852055 1 Reactome稳定的标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser_st_id?ST_ID=R-HSA-8852055.1 Reactome DB_ID: 113541 1 Reactome Database ID Release 77 8852128 数据库标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=8852128 Reactome R-HSA-8852128 2 Reactome稳定的标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser_st_id?ST_ID=R-HSA-8852128.2 8010951 Pubmed 1994 Nuclear localization of the ubiquitin-activating enzyme, E1, is cell-cycle-dependent Grenfell, S J Trausch-Azar, J S Handley-Gearhart, P M Ciechanover, A Schwartz, A L Biochem. J. 300:701-8 UBA1 conjugates ubiquitin to UBA1 in the nucleus UBA1 conjugates ubiquitin to UBA1 in the nucleus UBA1 is located in both the nucleus and cytoplasm (Grenfell 1994). Activation of ubiquitin by UBA1 proceeds through 3 steps: adenylation of ubiquitin, conjugation of ubiquitin from adenyl-ubiquitin to an internal cysteine residue of UBA1, and adenylation of a second molecule of ubiquitin. In the second step, the thiol group of an internal cysteine residue of UBA1 attacks the acyl-adenyl bond of the C-terminal glycine of adenylated ubiquitin, resulting in a thioester bond between the cysteine residue and the C-terminal glycine of ubiquitin (Jin et al. 2007, inferred from the rabbit homologue in Haas et al. 1982, Hershko et al. 1983, reviewed in Groettrup et al. 2008). Authored: May, Bruce, 2016-01-11 Reviewed: Azevedo, Jorge E, 2016-08-11 Edited: May, Bruce, 2016-01-11 Reactome DB_ID: 8852055 1 Reactome DB_ID: 164121 1 Reactome DB_ID: 8852095 1 Ub-Cys632-UBA1 [nucleoplasm] Ub-Cys632-UBA1 Reactome DB_ID: 8852116 1 2 EQUAL 1058 EQUAL Converted from EntitySet in Reactome Reactome DB_ID: 68524 1 Reactome Database ID Release 77 8852095 数据库标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=8852095 Reactome R-HSA-8852095 2 Reactome稳定的标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser_st_id?ST_ID=R-HSA-8852095.2 Reactome Database ID Release 77 8852136 数据库标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=8852136 Reactome R-HSA-8852136 1 Reactome稳定的标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser_st_id?ST_ID=R-HSA-8852136.1 Ub-Cys632-UBA1 adenylates ubiquitin in the nucleus Ub-Cys632-UBA1 adenylates ubiquitin in the nucleus UBA1 is located in both the cytoplasm and nucleus (Grenfell et al. 1994). Activation of ubiquitin by UBA1 proceeds through 3 steps: adenylation of ubiquitin, conjugation of ubiquitin from adenyl-ubiquitin to an internal cysteine residue of UBA1, and adenylation of a second molecule of ubiquitin. In the third step, the UBA1-ubiquitin conjugate adenylates the C-terminal glycine of a second ubiquitin molecule. This results in UBA1 loaded with 2 ubiquitin molecules, one of which is conjugated to an internal cysteine residue of UBA1 and one of which is adenylated and non-covalently bound to UBA1 (Jin et al. 2007, inferred from the rabbit homologue in Haas et al. 1982, Hershko et al. 1983, reviewed in Groettrup et al. 2008) Authored: May, Bruce, 2016-01-11 Reviewed: Azevedo, Jorge E, 2016-08-11 Edited: May, Bruce, 2016-01-11 Reactome DB_ID: 8852095 1 Reactome DB_ID: 29358 1 Converted from EntitySet in Reactome Reactome DB_ID: 68524 1 Reactome DB_ID: 8852059 1 Ub-Cys632-UBA1:泛素亚丁湾ylate [nucleoplasm] Ub-Cys632-UBA1:泛素亚丁湾ylate Converted from EntitySet in Reactome Reactome DB_ID: 8852106 1 Reactome DB_ID: 8852095 1 Reactome Database ID Release 77 8852059 数据库标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=8852059 Reactome R-HSA-8852059 1 Reactome稳定的标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser_st_id?ST_ID=R-HSA-8852059.1 Reactome DB_ID: 113541 1 Reactome Database ID Release 77 8852131 数据库标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=8852131 Reactome R-HSA-8852131 2 Reactome稳定的标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser_st_id?ST_ID=R-HSA-8852131.2 UBA1 conjugates ubiquitin to nuclear E2 enzymes UBA1 conjugates ubiquitin to nuclear E2 enzymes In the nucleus (Grenfell et al. 1994), the UBA1-ubiquitin thiol ester conjugate transfers ubiquitin from UBA1 to an internal cysteine residue of the E2 enzyme, forming a thiol ester conjugate between ubiquitin and the cysteine residue of E2 (Jin et al. 2007, inferred from the rabbit homologue in Hershko et al. 1983, reviewed in Groettrup et al. 2008). The E2 then disengages from UBA1 (Eletr et al. 2005). Authored: May, Bruce, 2016-01-11 Reviewed: Azevedo, Jorge E, 2016-08-11 Edited: May, Bruce, 2016-01-11 Reactome DB_ID: 8852059 1 Converted from EntitySet in Reactome Reactome DB_ID: 8852109 1 E2 ubiquitin conjugating enzyme (UBA1 substrate) [nucleoplasm] Converted from EntitySet in Reactome. Each synonym is a name of a PhysicalEntity, and each XREF points to one PhysicalEntity CDC34 [nucleoplasm] UBE2E3 [nucleoplasm] UBE2B [nucleoplasm] UBE2L3 [nucleoplasm] UBE2E1 [nucleoplasm] UBE2D2 [nucleoplasm] UBE2S [nucleoplasm] UBE2A [nucleoplasm] UBE2T(νcleoplasm] UBE2C [nucleoplasm] UBE2W [nucleoplasm] UniProt P63146 UniProt P51965 UniProt P49459 UniProt Q9NPD8 UniProt Q96B02 Reactome DB_ID: 8852055 1 Reactome DB_ID: 8864169 1 Ub:E2 (UBA1 substrate) [nucleoplasm] Ub:E2 (UBA1 substrate) Converted from EntitySet in Reactome Reactome DB_ID: 8864197 1 Ub-conjugated E2 enzyme (UBA1 substrate) [nucleoplasm] Converted from EntitySet in Reactome. Each synonym is a name of a PhysicalEntity, and each XREF points to one PhysicalEntity Ub-C91-UBE2W [nucleoplasm] Ub-C88-UBE2B [nucleoplasm] Ub-C131-UBE2E1 [nucleoplasm] Ub-C145-UBE2E3 [nucleoplasm] Ub-C86-UBE2L3 [nucleoplasm] Ub-C95-UBE2S [nucleoplasm] Ub-C88-UBE2A [nucleoplasm] Ub-C86-UBE2T [nucleoplasm] Ub-C85-UBE2D2 [nucleoplasm] Ub-C114-UBE2C [nucleoplasm] Ub-C93-CDC34 [nucleoplasm] Converted from EntitySet in Reactome Reactome DB_ID: 68524 1 Reactome Database ID Release 77 8864169 数据库标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=8864169 Reactome R-HSA-8864169 2 Reactome稳定的标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser_st_id?ST_ID=R-HSA-8864169.2 Reactome Database ID Release 77 8852130 数据库标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=8852130 Reactome R-HSA-8852130 1 Reactome稳定的标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser_st_id?ST_ID=R-HSA-8852130.1 Reactome Database ID Release 77 8866652 数据库标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=8866652 Reactome R-HSA-8866652 2 Reactome稳定的标识符。使用这个URL连接到the web page of this instance in Reactome: //www.joaskin.com/cgi-bin/eventbrowser_st_id?ST_ID=R-HSA-8866652.2 24457024 Pubmed 2014 Perilous journey: a tour of the ubiquitin-proteasome system Kleiger, Gary Mayor, Thibault Trends Cell Biol. 24:352-9 19940261 Pubmed 2010 The family of ubiquitin-conjugating enzymes (E2s): deciding between life and death of proteins van Wijk, SJ timmer, HT FASEB J 24:981-93 27002219 Pubmed 2016 E2 enzymes: more than just middle men Stewart, Mikaela D Ritterhoff, Tobias Klevit, Rachel E Brzovic, Peter S Cell Res. 26:423-40 GO 0016567 GO biological process