BioPAX通路转换从“BOTA HC输送BOTA LC从靶细胞突触小泡膜到胞浆”在Reactome数据库。
BOTA HC输送BOTA LC从靶细胞突触小泡膜到胞浆
BOTA HC输送BOTA LC从靶细胞突触小泡膜到胞浆
酸化,在胞吞作用正常步骤使在键合的重链的肉毒毒素二硫化物类型A的构象变化 - 轻链的异源二聚体(“迪辰”)(BOTA HC:LC)它包含,允许毒素的HC一部分用作信道,通过该其LC部分被挤压成神经元胞质溶胶。的HC - LC二硫键被裂解(Koriazova&2003 Montal; Montal 2010)。体外最近的研究表明,与毒素相关GT1b神经节苷脂可能在这一过程中发挥作用(Sun等2012)。
创作:鲁帕,S,Gopinathrao,G,2006-06-15 10时36分11秒
来自:Ichtchenko,K,2007-08-03十八点17分25秒
来自:Thirunavukkarasu,Nagarajan,夏尔马,沙市,情节中字
Reactome DB_ID:5244507
1
突触小泡膜
走
0030672
BOTA HC:LC二聚体:SV2:GT1b [突触小泡膜]
博塔HC:LC二聚体:SV2:GT1b
的BoNT / A:SV2:GT1b
从EntitySet的在Reactome转换
Reactome DB_ID:5324682
1
SV2A,B,C [突触小泡膜]
从EnticeSet转换在反应中。每个同义词是身份有力的名称,每个XREF都指向一个身体性感性
SV2B [突触小泡膜]
SV2A [突触小泡膜]
SV2C [突触小泡膜]
反应
//www.joaskin.com
HOMO SAPIENS.
NCBI分类学
9606.
uniprot.
Q7L1i2.
uniprot.
Q7L0J3
uniprot.
Q496J9
Reactome DB_ID:5244439
1
神经节苷脂GT1b [飞燕:60913]
神经节苷脂GT1b
GT1b神经节苷脂(C36)
Chebi.
60913
Reactome DB_ID:5228950
1
BOTA HC:LC二聚体[突触小泡膜]
博塔HC:LC二聚体
的BoNT / A HC:LC二聚体
Reactome DB_ID:190036
1
BOTA LC:Zn2 +的[突触小泡膜]
博塔LC:Zn2 +的
的BoNT轻链A型
的BoNT / A LC:Zn2 +的二硫键连接的
Reactome DB_ID:9616804
1
UniProt的:P0DPI0博塔
博塔
博塔
ATX
邦特
酶活性调节的内毒素是由叠氮化物或二硝基苯酚或在4摄氏度抑制(PUBMED:6694738)。动力素(DNM)抑制剂废除毒素摄取(搜索PubMed:21832053).SUBUNIT异源二聚体;的轻链(LC)和重链(HC)的二硫键连接的异源二聚体(PUBMED:7578132)。与糖基化的宿主突触小泡糖蛋白SV2A,SV2B和SV2C相互作用其用作辅助受体(PUBMED:16543415,PUBMED:18815274,PUBMED:19650874,PUBMED:24240280,PUBMED:27313224)。在SV2C“的Asn-559”的糖基化有助于在亲和力这种相互作用(PUBMED:27313224)的12倍的增加。与高水平K(+)导致受体曝光的更高水平的靶组织的去极化(PUBMED:16543415)。体外此外神经节苷脂的增加SV2毒素相互作用(搜索PubMed:16543415)。形成高度互锁异源二聚体与NTNHA在pH 6.0但不在称为微创官能祖毒素复合物(M-PTC)的pH 7.5(PUBMED:22363010)。所述PTC被认为保护毒素在宿主酸性胃肠道,促进跨在中性pH下的肠屏障和释放胞转如在血流中(PUBMED:22363010)被发现柳炳祥李海林在培养的细菌,在后期指数生长第一检测(17小时),在24-25小时达到最大水平,并通过神经细胞保持5天几乎恒定的(在蛋白质水平).BIOTECHNOLOGY动力素抑制剂缓慢毒素摄取,并且可以用于延长治疗窗口的antitoxins.BIOTECHNOLOGY代用 RBD by other proteins (such as wheat germ agglutinin) allows the rest of the toxin to be taken up by other cell types, and can be used for investigating synaptic vesicle docking-dependent processes in BoNT resistant cells (PubMed:10768948). LC retains protease activity (PubMed:10768948, PubMed:19351593).BIOTECHNOLOGY Isolated receptor-binding domain (RBD) can be used as a vaccine; a mutated form that is transcytosed into the general circulation but does not enter nerve cells (Leu-1266-1267-Ser) is as efficient as wild-type RBD (PubMed:21106906).PHARMACEUTICAL Available under the name Botox (onabotulinumtoxinA, Allergan), Dysport (abobotulinumtoxinA, Ipsen Biopharmaceuticals) and Xeomin (incobotulinumtoxinA, Merz Pharmaceuticals) for the treatment of strabismus and blepharospasm associated with dystonia and cervical dystonia. Also used for the treatment of hemifacial spasm and a number of other neurological disorders characterized by abnormal muscle contraction. It is also used cosmetically to smooth facial wrinkles.MISCELLANEOUS There are seven antigenically distinct forms of botulinum neurotoxin: Types A, B, C, D, E, F, and G; new subtypes are quite frequent.MISCELLANEOUS Types A, B and E are the most frequent cause of adult human foodborne botulism; type A is the most severe, while type E has the shortest incubation period (PubMed:1431246).MISCELLANEOUS Neurotoxin type A is released from bacteria in the two-chain form (PubMed:6370252, PubMed:2126206).MISCELLANEOUS Botulism poisoning is usually food-borne, either by ingesting toxin or bacterial-contaminated food, or less frequently by inhalation poisoning. In both cases the neurotoxin binds to the apical surface of epithelial cells in the gut or airway. Toxin undergoes receptor-mediated endocytosis (using a different receptor than on target nerve cells), transcytosis across the epithelial cells and release into the general circulation. Once in the general circulation it binds to its target cells.SIMILARITY Belongs to the peptidase M27 family.CAUTION Contradictory results show that only SV2C is the receptor; in these experiments gangliosides do not improve toxin-coreceptor interaction (PubMed:16545378).
Clostridium botulinum.
NCBI分类学
1491.
uniprot.
P0DPI0
链间交联通过L-胱氨酸(交联)在430和454
430.
平等的
L-胱氨酸(交联)
Chebi.
50058
修改
链坐标
2
平等的
448.
平等的
Reactome DB_ID:5228942
1
锌(2+)[飞燕:29105]
锌(2+)
Chebi.
29105
反应数据库ID版本77
190036
数据库标识符。使用此URL连接到该实例的网页中Reactome://www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=190036
反应
R-CBO-190036
9.
Reactome稳定标识符。使用此URL连接到该实例的网页中Reactome://www.joaskin.com/cgi-bin/eventbrowser_st_id?ST_ID=R-CBO-190036.9
Reactome DB_ID:9616795
1
链间交联通过L-胱氨酸(交联)在454和430
454.
平等的
449.
平等的
1296.
平等的
反应数据库ID版本77
5228950
数据库标识符。使用此URL连接到该实例的网页中Reactome://www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=5228950
反应
R-CBO-5228950
9.
Reactome稳定标识符。使用此URL连接到该实例的网页中Reactome://www.joaskin.com/cgi-bin/eventbrowser_st_id?ST_ID=R-CBO-5228950.9
反应数据库ID版本77
5244507
数据库标识符。使用此URL连接到该实例的网页中Reactome://www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=5244507
反应
R-HSA-5244507
9.
Reactome稳定标识符。使用此URL连接到该实例的网页中Reactome://www.joaskin.com/cgi-bin/eventbrowser_st_id?ST_ID=R-HSA-5244507.9
Reactome DB_ID:190017
1
cytosol.
走
0005829.
BOTA LC:Zn2 +的[胞质溶胶]
博塔LC:Zn2 +的
的BoNT轻链A型
Reactome DB_ID:29426
1
Reactome DB_ID:9616805
1
2
平等的
448.
平等的
反应数据库ID版本77
190017
数据库标识符。使用此URL连接到该实例的网页中Reactome://www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=190017
反应
R-CBO-190017
9.
Reactome稳定标识符。使用此URL连接到该实例的网页中Reactome://www.joaskin.com/cgi-bin/eventbrowser_st_id?ST_ID=R-CBO-190017.9
从EntitySet的在Reactome转换
Reactome DB_ID:5324682
1
Reactome DB_ID:5244439
1
Reactome DB_ID:9616778
1
449.
平等的
1296.
平等的
PHYSIOL-LEFT-TO-RIGHT
激活
Reactome DB_ID:5244507
走
0008320
GO分子功能
反应数据库ID版本77
5244412
数据库标识符。使用此URL连接到该实例的网页中Reactome://www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=5244412
反应数据库ID版本77
5244428
数据库标识符。使用此URL连接到该实例的网页中Reactome://www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=5244428
反应
R-HSA-5244428
6.
Reactome稳定标识符。使用此URL连接到该实例的网页中Reactome://www.joaskin.com/cgi-bin/eventbrowser_st_id?ST_ID=R-HSA-5244428.6
20233039
PubMed.
2010年
肉毒杆菌神经毒素:蛋白质设计的奇迹
Montal,毛
安努。Rev. Biochem。79:591-617
12459720
PubMed.
2003年
通过重链通道肉毒杆菌神经毒素的轻链蛋白酶易位
Koriazova,莉利亚ķ
Montal,毛
纳特结构生物学10:13-8
22720883
PubMed.
2012年
肉毒神经毒素B和E易位以不同的速率和展览发散响应GT1b和低pH值
太阳,石湖
特普,威廉姆·H·
约翰逊,埃里克·一
查普曼,埃德温 - [R
生物化学51:5655-62
激活
反应数据库ID版本77
5244431
数据库标识符。使用此URL连接到该实例的网页中Reactome://www.joaskin.com/cgi-bin/eventbrowser?DB=gk_current&ID=5244431
反应
R-HSA-5244431
1
Reactome稳定标识符。使用此URL连接到该实例的网页中Reactome://www.joaskin.com/cgi-bin/eventbrowser_st_id?ST_ID=R-HSA-5244431.1
Reactome DB_ID:5244410
突触小泡腔
走
0034592
昌[飞燕:15378]
昌
Chebi.
15378